Activating Compound | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | inhibition | Rattus norvegicus |
General Stability | Organism |
---|---|
dithiothreitol, 1 mM, stabilizes | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
histamine | - |
Rattus norvegicus | |
iodoacetamide | complete inhibition at 0.01 mM | Rattus norvegicus | |
Methylhistamine | - |
Rattus norvegicus | |
methylthioadenosine | - |
Rattus norvegicus | |
p-hydroxymercuribenzoate | complete inhibition at 0.1 mM | Rattus norvegicus | |
S-adenosylhomocysteine | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0061 | - |
histamine | - |
Rattus norvegicus | |
0.0071 | - |
S-adenosyl-L-methionine | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Rattus norvegicus |
36000 | - |
gel filtration | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
renal enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 10 mM potassium phosphate buffer, pH 7.4, 10% glycerol, 1 mM dithiothreitol, 8 months, 16% loss of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + histamine | - |
Rattus norvegicus | S-adenosyl-L-homocysteine + Ntau-methylhistamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
90 min, 1 mM dithiothreitol, stable, 40% loss of activity without addition of dithiothreitol | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | 9.4 | - |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.3 | 10.3 | about 50% of maximum activity at pH 7.3 and pH 10.3 | Rattus norvegicus |