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Literature summary for 2.1.1.77 extracted from

  • Mishra, P.K.K.; Gattani, A.; Mahawar, M.
    Isolation and identification of protein L-isoaspartate-O-methyltransferase (PIMT) interacting proteins in Salmonella Typhimurium (2020), Curr. Microbiol., 77, 695-701 .
    View publication on PubMed
Search for more literature for 2.1.1.77

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli NEB5-alpha cells and T7 lys gamma cells Salmonella enterica subsp. enterica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate Salmonella enterica subsp. enterica
-
 S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica
-
 serovar Typhimurium, E-2375
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Salmonella enterica subsp. enterica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + protein L-isoaspartate
-
 Salmonella enterica subsp. enterica S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 26000, SDS-PAGE Salmonella enterica subsp. enterica

Synonyms

Synonyms Comment Organism
PIMT
-
 Salmonella enterica subsp. enterica
protein L-isoaspartate-O-methyltransferase
-
 Salmonella enterica subsp. enterica

Expression

Organism Comment Expression
Salmonella enterica subsp. enterica temperature (42 °C) induces elevation in enzyme expression level up

General Information

General Information Comment Organism
physiological function the enzyme is crucial for survival, virulence and colonization of Salmonella Typhimurium in poultry Salmonella enterica subsp. enterica