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Literature summary for 2.1.1.75 extracted from

  • Liu, H.; Xu, R.X.; Gao, S.; Cheng, A.X.
    The functional characterization of a site-specific apigenin 4'-O-methyltransferase synthesized by the liverwort species Plagiochasma appendiculatum (2017), Molecules, 22, 759 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.75

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Plagiochasma appendiculatum

Inhibitors

Inhibitors Comment Organism Structure
luteolin
-
 Plagiochasma appendiculatum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.031
-
 apigenin at pH 7.5 and 37°C Plagiochasma appendiculatum
0.0521
-
 luteolin at pH 7.5 and 37°C Plagiochasma appendiculatum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not affected by Mg2+ Plagiochasma appendiculatum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + apigenin Plagiochasma appendiculatum
-
 S-adenosyl-L-homocysteine + acacetin
-
 ?

Organism

Organism UniProt Comment Textmining
Plagiochasma appendiculatum A0A1X9Y2Y6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA Sefinose column chromatography Plagiochasma appendiculatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with baicalein, acacetin, caffeic acid, caffeoyl aldehyde, caffeoyl alcohol, 5-hydroxyconiferyl aldehyde, and 5-hydroxyconiferyl alcohol Plagiochasma appendiculatum ?
-
-
S-adenosyl-L-methionine + apigenin
-
 Plagiochasma appendiculatum S-adenosyl-L-homocysteine + acacetin
-
 ?
S-adenosyl-L-methionine + apigenin 100% activity Plagiochasma appendiculatum S-adenosyl-L-homocysteine + acacetin
-
 ?
S-adenosyl-L-methionine + genistein less than 1% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?
S-adenosyl-L-methionine + genkwanin about 10% activity compared to apigenin Plagiochasma appendiculatum S-adenosyl-L-homocysteine + apigenin-7,4'-dimethyl ether
-
 ?
S-adenosyl-L-methionine + kaempferol about 10% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?
S-adenosyl-L-methionine + luteolin about 35% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?
S-adenosyl-L-methionine + naringenin less than 1% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?
S-adenosyl-L-methionine + quercetin about 10% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?
S-adenosyl-L-methionine + scutellarein about 18% activity compared to apigenin Plagiochasma appendiculatum ?
-
 ?

Synonyms

Synonyms Comment Organism
F4'OMT
-
 Plagiochasma appendiculatum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Plagiochasma appendiculatum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.08
-
 luteolin at pH 7.5 and 37°C Plagiochasma appendiculatum
0.117
-
 apigenin at pH 7.5 and 37°C Plagiochasma appendiculatum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Plagiochasma appendiculatum

pH Range

pH Minimum pH Maximum Comment Organism
7.5 9 the enzyme shows high catalytic activity over the pH range 7.5-9.0 Plagiochasma appendiculatum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2928
-
 luteolin at pH 7.5 and 37°C Plagiochasma appendiculatum