Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, for wild type, enzyme using 0.2 M ammonium acetate, 22% (w/v) PEG 3350, and 0.1 M HEPES pH 7.5, while for mutant enzymes R37K and Y139F using 0.1 M HEPES pH 7.5, 4% polyethylene glycol 8000, and either 4% or 8% ethylene glycol, respectively | Mycobacterium tuberculosis |
Protein Variants | Comment | Organism |
---|---|---|
R37E | the mutant exhibits a 5fold lower affinity for the methylated duplex DNA compared to the wild type enzyme | Mycobacterium tuberculosis |
R37K | the variant performs a sub-optimal alkylated-DNA repair in vitro compared to the wild type enzyme | Mycobacterium tuberculosis |
R37L | the variant performs a sub-optimal alkylated-DNA repair in vitro compared to the wild type enzyme | Mycobacterium tuberculosis |
Y139F | the mutant exhibits a 10fold lower affinity for the methylated duplex DNA compared to the wild type enzyme | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + protein L-cysteine | Mycobacterium tuberculosis | - |
DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | P9WJW5 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 200 gel filtration | Mycobacterium tuberculosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA (containing 6-O-methylguanine) + protein L-cysteine | - |
Mycobacterium tuberculosis | DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
O6-methylguanine-DNA methyltransferase | - |
Mycobacterium tuberculosis |
OGT | - |
Mycobacterium tuberculosis |