Activating Compound | Comment | Organism | Structure |
---|---|---|---|
nsp10 protein | nsp16 requires non-structural protein nsp10 as a stimulatory factor to execute its methyltransferase activity by binding both S-adenosyl-L-methionine and RNA substrate | Severe acute respiratory syndrome-related coronavirus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Severe acute respiratory syndrome-related coronavirus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using in 0.1 M MES, pH 5.0, 2 M NaCl, 0.1 M NaH2PO4, 0.1 M KH2PO4 after 24 h at 25°C | Severe acute respiratory syndrome-related coronavirus |
Protein Variants | Comment | Organism |
---|---|---|
G70A | the mutant shows reduced activity compared to the wild type | Severe acute respiratory syndrome-related coronavirus |
H83A/P84A | inactive | Severe acute respiratory syndrome-related coronavirus |
K93A | the mutant shows reduced activity compared to the wild type | Severe acute respiratory syndrome-related coronavirus |
Y76A/C77A/R78A | inactive | Severe acute respiratory syndrome-related coronavirus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Severe acute respiratory syndrome-related coronavirus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Severe acute respiratory syndrome-related coronavirus | G8H306 | nsp16 | - |
Severe acute respiratory syndrome-related coronavirus WHU | G8H306 | nsp16 | - |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 200 gel filtration | Severe acute respiratory syndrome-related coronavirus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme does not methylate m7G(5')pppG-RNA | Severe acute respiratory syndrome-related coronavirus | ? | - |
? | |
additional information | the enzyme does not methylate m7G(5')pppG-RNA | Severe acute respiratory syndrome-related coronavirus WHU | ? | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppA-RNA | - |
Severe acute respiratory syndrome-related coronavirus | S-adenosyl-L-homocysteine + m7G(5')pppRA-RNA | - |
? | |
S-adenosyl-L-methionine + m7G(5')pppA-RNA | - |
Severe acute respiratory syndrome-related coronavirus WHU | S-adenosyl-L-homocysteine + m7G(5')pppRA-RNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Severe acute respiratory syndrome-related coronavirus |
Synonyms | Comment | Organism |
---|---|---|
2'-O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
2'O-MTase | - |
Severe acute respiratory syndrome-related coronavirus |
nsp16 | - |
Severe acute respiratory syndrome-related coronavirus |
nsp16/nsp10 protein complex | - |
Severe acute respiratory syndrome-related coronavirus |