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Literature summary for 2.1.1.5 extracted from
- Human hepatic methionine biosynthesis. Purification and characterization of betaine:homocysteine S-methyltransferase (1982), J. Biol. Chem., 257, 14944-14948.
General Stability
| General Stability | Organism |
|---|---|
| divalent cations and 2-mercaptoethanol do not stabilize during purification | Homo sapiens |
| substrates stabilize during purification | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3,3-Dimethylbutyrate | competitive to betaine | Homo sapiens |  |
| Isovalerate | competitive to betaine | Homo sapiens | |
| additional information | not inhibitory: methionine or ethionine; product inhibition | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.1 | - |
betaine | - |
Homo sapiens | |
| 0.12 | - |
L-homocysteine | - |
Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Homo sapiens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid composition | Homo sapiens |
| 45000 | - |
6 * 45000, SDS-PAGE | Homo sapiens |
| 270000 | - |
gel filtration | Homo sapiens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two forms of the enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, many months in 50% v/v glycerol | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N,N,N-trimethylglycine + L-homocysteine | - |
Homo sapiens | N,N-dimethylglycine + L-methionine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | 6 * 45000, SDS-PAGE | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
significant heat stability | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
above, 4 h stable at 37°C | Homo sapiens |
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