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Literature summary for 2.1.1.379 extracted from

  • Radle, M.I.; Miller, D.V.; Laremore, T.N.; Booker, S.J.
    Methanogenesis marker protein 10 (Mmp10) from Methanosarcina acetivorans is a radical S-adenosylmethionine methylase that unexpectedly requires cobalamin (2019), J. Biol. Chem., 294, 11712-11725 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanosarcina acetivorans

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ the enzyme contain 5.09 iron ions per polypeptide Methanosarcina acetivorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor Methanosarcina acetivorans
-
 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina acetivorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cobalt-Talon resin column chromatography Methanosarcina acetivorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + a [methyl coenzyme-M reductase]-L-arginine285 + reduced acceptor
-
 Methanosarcina acetivorans S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + a [methyl coenzyme-M reductase]-(5S)-C-methyl-L-arginine285 + acceptor
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Methanosarcina acetivorans
? x * 46300, calculated from amino acid sequence Methanosarcina acetivorans

Synonyms

Synonyms Comment Organism
methanogenesis marker protein 10
-
 Methanosarcina acetivorans
MMP10
-
 Methanosarcina acetivorans

Cofactor

Cofactor Comment Organism Structure
Cobalamin required for activity Methanosarcina acetivorans
S-adenosyl-L-methionine
-
 Methanosarcina acetivorans