Cloned (Comment) | Organism |
---|---|
glutathione-S-transferase-fusion protein, SET gene | Homo sapiens |
glutathione-S-transferase-fusion protein, SET gene | Drosophila elegans |
Protein Variants | Comment | Organism |
---|---|---|
H299A | overlap PCR, destroyed SET gene, no activity remaining | Drosophila elegans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Homo sapiens |
45000 | - |
SDS-PAGE | Drosophila elegans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | - |
? | - |
? | |
additional information | Homo sapiens | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | ? | - |
? | |
additional information | Drosophila elegans | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | ? | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Drosophila melanogaster | - |
S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Homo sapiens | Lys20 of histone 4, SET8 | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Drosophila elegans | Lys20 of histone 4, SET8 | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila elegans | - |
- |
- |
Drosophila melanogaster | - |
SET8 sequence | - |
Homo sapiens | Q9NQR1 | HeLa cells | - |
Homo sapiens | Q9NQR1 | SET8 sequence | - |
Purification (Comment) | Organism |
---|---|
partial | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | amino acid sequence comparison | Homo sapiens | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | amino acid sequence comparison | Drosophila elegans | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence | Drosophila melanogaster | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence | Homo sapiens | |
S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | enzyme is encoded on genes containing the SET-domain sequence, substrate specificity is connected to SET sequence | Drosophila elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | - |
Drosophila melanogaster | ? | - |
? | |
additional information | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | Homo sapiens | ? | - |
? | |
additional information | histone methylation has significant effects on heterochromatin formation and transcriptional regulation | Drosophila elegans | ? | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | - |
Drosophila melanogaster | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | - |
Drosophila elegans | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | high substrate and methylation site specificity | Drosophila melanogaster | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | high substrate and methylation site specificity | Homo sapiens | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Lys20 of histone 4 | Drosophila melanogaster | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Lys20 of histone 4 | Homo sapiens | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Lys20 of histone 4, SET8 | Homo sapiens | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? | |
S-adenosyl-L-methionine + histone L-lysine | Lys20 of histone 4, SET8 | Drosophila elegans | S-adenosyl-L-homocysteine + histone N6-methyl-L-lysine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Homo sapiens |
monomer | 1 * 45000, SDS-PAGE | Drosophila elegans |