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Literature summary for 2.1.1.359 extracted from

  • Eram, M.S.; Bustos, S.P.; Lima-Fernandes, E.; Siarheyeva, A.; Senisterra, G.; Hajian, T.; Chau, I.; Duan, S.; Wu, H.; Dombrovski, L.; Schapira, M.; Arrowsmith, C.H.; Vedadi, M.
    Trimethylation of histone H3 lysine 36 by human methyltransferase PRDM9 protein (2014), J. Biol. Chem., 289, 12177-12188 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.359

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol maximal enzyme activity at 10 mM Homo sapiens
DMSO maximal activity at 0.16% (v/v) Homo sapiens
TCEP maximal enzyme activity at 0.6 mM Homo sapiens
Triton X-100 maximal activity at 0.004% (v/v) Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pRARE2-V2R cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
NaCl enzyme activity is abolished at NaCl concentrations above 125 mM Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
 [histone H3]-L-lysine36 at pH 8.5 and 23°C Homo sapiens
0.0024
-
 [histone H3]-N6-methyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens
0.0025
-
 [histone H3]-N6,N6-dimethyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens
0.062
-
 S-adenosyl-L-methionine with [histone H3]-N6,N6-dimethyl-L-lysine36 as cosubstrate, at pH 8.5 and 23°C Homo sapiens
0.087
-
 S-adenosyl-L-methionine with [histone H3]-L-lysine36 as cosubstrate, at pH 8.5 and 23°C Homo sapiens
0.13
-
 S-adenosyl-L-methionine with [histone H3]-N6-methyl-L-lysine36 as cosubstrate, at pH 8.5 and 23°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 Homo sapiens overall reaction 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
additional information Homo sapiens no activity with unmethylated/methylated [histone H3]-L-lysine27 and [histone H3]-L-lysine20 as well as trimethylated [histone H3]-L-lysine4 and [histone H3]-L-lysine36 peptides ?
-
 ?
additional information Homo sapiens the enzyme also efficiently trimethylates [histone H3]-L-lysine4 ?
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine36 Homo sapiens
-
 S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine36 Homo sapiens highest activity S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine36 Homo sapiens
-
 S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9NQV7
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-Trap column chromatography, Source30Q column chromatography, and Superdex S200 gel filtration Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + a [histone H3]-L-lysine36 overall reaction Homo sapiens 3 S-adenosyl-L-homocysteine + a [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
additional information no activity with unmethylated/methylated [histone H3]-L-lysine27 and [histone H3]-L-lysine20 as well as trimethylated [histone H3]-L-lysine4 and [histone H3]-L-lysine36 peptides Homo sapiens ?
-
 ?
additional information the enzyme also efficiently trimethylates [histone H3]-L-lysine4 Homo sapiens ?
-
 ?
S-adenosyl-L-methionine + [histone H3]-L-lysine36
-
 Homo sapiens S-adenosyl-L-homocysteine + [histone H3]-N6-methyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6,N6-dimethyl-L-lysine36 highest activity Homo sapiens S-adenosyl-L-homocysteine + [histone H3]-N6,N6,N6-trimethyl-L-lysine36
-
 ?
S-adenosyl-L-methionine + [histone H3]-N6-methyl-L-lysine36
-
 Homo sapiens S-adenosyl-L-homocysteine + [histone H3]-N6,N6-dimethyl-L-lysine36
-
 ?

Synonyms

Synonyms Comment Organism
PR domain-containing protein 9
-
 Homo sapiens
Prdm9
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.389
-
 [histone H3]-N6-methyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens
2.083
-
 [histone H3]-L-lysine36 at pH 8.5 and 23°C Homo sapiens
5.278
-
 [histone H3]-N6,N6-dimethyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
577.8
-
 [histone H3]-N6-methyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens
1388
-
 [histone H3]-L-lysine36 at pH 8.5 and 23°C Homo sapiens
2111
-
 [histone H3]-N6,N6-dimethyl-L-lysine36 at pH 8.5 and 23°C Homo sapiens