BRENDA - Enzyme Database
show all sequences of 2.1.1.279

Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain

Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.; Plant Physiol. 149, 384-394 (2009)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Pimpinella anisum
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
2.5 mM, 97% inhibition
Pimpinella anisum
Fe2+
2.5 mM, 47% inhibition
Pimpinella anisum
Mn2+
2.5 mM, 35% inhibition
Pimpinella anisum
additional information
no inhibitory effect on the activity is observed by incubation with 2.5 mM K+, Na+, Ca2+, and Mg2+
Pimpinella anisum
Zn2+
2.5 mM, 97% inhibition
Pimpinella anisum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0193
-
isoeugenol
pH 7.5, 25C
Pimpinella anisum
0.0545
-
S-adenosyl-L-methionine
pH 7.5, 25C
Pimpinella anisum
Organism
Organism
UniProt
Commentary
Textmining
Pimpinella anisum
B8RCD3
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
S-adenosyl-L-methionine + isoeugenol = S-adenosyl-L-homocysteine + isomethyleugenol
(2)
Pimpinella anisum
S-adenosyl-L-methionine + trans-anol = S-adenosyl-L-homocysteine + trans-anethole
(1)
Pimpinella anisum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
bud
-
Pimpinella anisum
-
flower
-
Pimpinella anisum
-
fruit
the gene encoding AIMT1 is expressed throughout the plant and the transcript level is highest in developing fruits
Pimpinella anisum
-
leaf
young, very low activity in mature leaves
Pimpinella anisum
-
root
-
Pimpinella anisum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
the enzyme does not have activity with eugenol or chavicol
700822
Pimpinella anisum
?
-
-
-
-
S-adenosyl-L-methionine +
shows a 10fold preference for isoeugenol over eugenol The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + chavicol
the enzyme shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
the enzyme prefers isoeugenol to t-anol by a factor of 2. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
-
?
S-adenosyl-L-methionine + trans-anol
trans-anol i.e. 4-[(1E)-prop-1-en-1-yl]phenol. The enzyme prefers isoeugenol to t-anol by a factor of 2. It shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + trans-anethole
trans-anethole i.e. 1-methoxy-4-[(1E)-prop-1-en-1-yl]benzene
-
-
?
Synonyms
Synonyms
Commentary
Organism
AIMT1
-
Pimpinella anisum
S-adenosyl-L-methionine:t-anol/isoeugenol O-methyltransferase
-
Pimpinella anisum
t-anol O-methyltransferase
-
Pimpinella anisum
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
37
30 min, stable
Pimpinella anisum
50
-
30 min, 12% loss of activity
Pimpinella anisum
65
-
30 min, less than 5% of activity remaining
Pimpinella anisum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.015
-
isoeugenol
pH 7.5, 25C
Pimpinella anisum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
Tris buffer
Pimpinella anisum
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
9
active in the range
Pimpinella anisum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Pimpinella anisum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
2.5 mM, 97% inhibition
Pimpinella anisum
Fe2+
2.5 mM, 47% inhibition
Pimpinella anisum
Mn2+
2.5 mM, 35% inhibition
Pimpinella anisum
additional information
no inhibitory effect on the activity is observed by incubation with 2.5 mM K+, Na+, Ca2+, and Mg2+
Pimpinella anisum
Zn2+
2.5 mM, 97% inhibition
Pimpinella anisum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0193
-
isoeugenol
pH 7.5, 25C
Pimpinella anisum
0.0545
-
S-adenosyl-L-methionine
pH 7.5, 25C
Pimpinella anisum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
bud
-
Pimpinella anisum
-
flower
-
Pimpinella anisum
-
fruit
the gene encoding AIMT1 is expressed throughout the plant and the transcript level is highest in developing fruits
Pimpinella anisum
-
leaf
young, very low activity in mature leaves
Pimpinella anisum
-
root
-
Pimpinella anisum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
the enzyme does not have activity with eugenol or chavicol
700822
Pimpinella anisum
?
-
-
-
-
S-adenosyl-L-methionine +
shows a 10fold preference for isoeugenol over eugenol The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + chavicol
the enzyme shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + isoeugenol
the enzyme prefers isoeugenol to t-anol by a factor of 2. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + isomethyleugenol
-
-
-
?
S-adenosyl-L-methionine + trans-anol
trans-anol i.e. 4-[(1E)-prop-1-en-1-yl]phenol. The enzyme prefers isoeugenol to t-anol by a factor of 2. It shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8
700822
Pimpinella anisum
S-adenosyl-L-homocysteine + trans-anethole
trans-anethole i.e. 1-methoxy-4-[(1E)-prop-1-en-1-yl]benzene
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
20
37
30 min, stable
Pimpinella anisum
50
-
30 min, 12% loss of activity
Pimpinella anisum
65
-
30 min, less than 5% of activity remaining
Pimpinella anisum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.015
-
isoeugenol
pH 7.5, 25C
Pimpinella anisum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
Tris buffer
Pimpinella anisum
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
9
active in the range
Pimpinella anisum
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.78
-
isoeugenol
pH 7.5, temperature not specified in the publication
Pimpinella anisum
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.78
-
isoeugenol
pH 7.5, temperature not specified in the publication
Pimpinella anisum
Other publictions for EC 2.1.1.279
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
700822
Koeduka
Biosynthesis of t-anethole in ...
Pimpinella anisum
Plant Physiol.
149
384-394
2009
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1
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5
2
-
-
-
-
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1
-
-
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2
-
5
-
-
5
-
3
-
-
3
1
1
1
-
-
-
-
-
-
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1
-
-
-
-
-
5
-
2
-
-
-
-
-
-
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5
-
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5
-
-
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3
1
1
1
-
-
-
-
-
-
1
1
726235
Gross
-
Biosynthesis of estragole and ...
Foeniculum vulgare
Plant Sci.
163
1047-1053
2002
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
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4
-
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1
1
3
1
1
-
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-
-
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-
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2
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1
1
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1
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4
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1
1
1
1
-
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1
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1
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