Literature summary for 2.1.1.277 extracted from

Luo, Z.W.; Cho, J.S.; Lee, S.Y.
Microbial production of methyl anthranilate, a grape flavor compound (2019), Proc. Natl. Acad. Sci. USA, 116, 10749-10756 .

Search for more literature for 2.1.1.277

Cloned(Commentary)

Cloned (Comment)	Organism
gene aamt1, recombinant expresion in Escherichia coli strain W3110 trpD9923 and Corynebacterium glutamicum strain ATCC 13032	Zea mays

Protein Variants

Protein Variants	Comment	Organism
additional information	metabolic engineering of Escherichia coli and Corynebacterium glutamicum strains by expression of plant enzyme anthranilic acid methyltransferase1 (AAMT1). Optimizing the key enzyme anthranilic acid (ANT) methyltransferase1 (AAMT1) expression, increasing the direct precursor ANT supply, and enhancing the intracellular availability and salvage of the cofactor S-adenosyl-L-methionine required by AAMT1, results in improved methyl anthranilate (MANT) production in both engineered microorganisms. In situ two-phase extractive fermentation using tributyrin as an extractant is developed to overcome MANT toxicity. Fed-batch cultures of the final engineered Escherichia coli and Corynebacterium glutamicum strains in two-phase cultivation mode lead to the production of 4.47 and 5.74 g/l MANT, respectively, in minimal media containing glucose. Metabolic network related to MANT biosynthesis from glucose in Escherichia coli and Corynebacterium glutamicum, as well as metabolic engineering strategies, methods, overview	Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + anthranilate	Zea mays	-	S-adenosyl-L-homocysteine + O-methyl anthranilate	-	?

Organism

Organism	UniProt	Comment	Textmining
Zea mays	D9J0Z7	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + anthranilate	-	Zea mays	S-adenosyl-L-homocysteine + O-methyl anthranilate	-	?

Synonyms

Synonyms	Comment	Organism
AAMT1	-	Zea mays
ANT methyltransferase1	-	Zea mays
anthranilic acid methyltransferase1	-	Zea mays

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Zea mays

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)