Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alamethicin | treatment of detached Arabidopsis thaliana leaves efficiently induced methyl salicylate but not methyl benzoate emission | Arabidopsis thaliana | |
insect herbivory | feeding by the larvae of the diamondback moth on rosette leaves of intact plants resultes in low methyl salicylate and very low methyl benzoate emission | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
coding regions of AlBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli | Arabidopsis lyrata |
coding regions of AtBSMT1 ligated into pCRT7/CT-TOPO TA vector for functional expression in Escherichia coli | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.065 | - |
benzoic acid | apparent Km-value, pH 7.7, 30°C | Arabidopsis thaliana | |
0.067 | - |
S-adenosyl-L-methionine | apparent Km-value, pH 7.7, 30°C | Arabidopsis thaliana | |
0.087 | - |
S-adenosyl-L-methionine | apparent Km-value, pH 7.7, 30°C | Arabidopsis lyrata | |
0.131 | - |
benzoic acid | apparent Km-value, pH 7.7, 30°C | Arabidopsis lyrata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43360 | - |
AlBSMT1 subunit, calculated from sequence | Arabidopsis lyrata |
43363 | - |
2 * 44000, SDSPAGE, 2 * 43363 calculated from sequence | Arabidopsis lyrata |
43365 | - |
2 * 44000, SDSPAGE, 2 * 43365 calculated from sequence | Arabidopsis thaliana |
43370 | - |
subunit, calculated from sequence | Arabidopsis thaliana |
44000 | - |
AlBSMT1 subunit mass by SDSPAGE | Arabidopsis lyrata |
44000 | - |
subunit mass by SDSPAGE | Arabidopsis thaliana |
91000 | - |
AlBSMT1 holoenzyme, estimated by size exclusion chromatography | Arabidopsis lyrata |
91000 | - |
holoenzyme, estimated by size exclusion chromatography | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis lyrata | Q6XMI1 | - |
- |
Arabidopsis thaliana | Q6XMI3 | - |
- |
Purification (Comment) | Organism |
---|---|
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography | Arabidopsis thaliana |
to near homogeneity in two steps, first using anion-exchange column diethylaminoethyl cellulose followed by Mono-Q anion-exchange chromatography | Arabidopsis lyrata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | AlBSMT1 transcript levels are, similarly to AtBSMT1 transcripts in Arabidopsis thaliana higher in flowers | Arabidopsis lyrata | - |
flower | highest levels of AtBSMT1 transcript | Arabidopsis thaliana | - |
leaf | detectable AlBSMT1 transcript levels | Arabidopsis lyrata | - |
leaf | lower levels of AtBSMT1 transcript | Arabidopsis thaliana | - |
additional information | little or no AtBSMT1 transcripts observed in roots or siliques of plants | Arabidopsis thaliana | - |
stem | lower levels of AtBSMT1 transcript | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | in contrast to AtBSMT1 no activity with 1 mM 3-hydroxybenzoic acid | Arabidopsis lyrata | ? | - |
? | |
additional information | The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase | Arabidopsis thaliana | ? | - |
? | |
additional information | The enzyme, which is found in flowering plants, also has the activity of EC 2.1.1.274, salicylate 1-O-methyltransferase | Arabidopsis lyrata | ? | - |
? | |
S-adenosyl-L-methionine + 3-hydroxybenzoic acid | 54% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl 3-hydroxybenzoate | - |
? | |
S-adenosyl-L-methionine + anthranilic acid | 32% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% | Arabidopsis lyrata | S-adenosyl-L-homocysteine + methyl anthranilate | - |
? | |
S-adenosyl-L-methionine + anthranilic acid | 35% relative activity at 1 mM methyl acceptor compared to activity with benzoic acid set at 100% | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl anthranilate | - |
? | |
S-adenosyl-L-methionine + benzoic acid | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl benzoate | - |
? | |
S-adenosyl-L-methionine + benzoic acid | - |
Arabidopsis lyrata | S-adenosyl-L-homocysteine + methyl benzoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 44000, SDSPAGE, 2 * 43363 calculated from sequence | Arabidopsis lyrata |
homodimer | 2 * 44000, SDSPAGE, 2 * 43365 calculated from sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AlBSMT1 | - |
Arabidopsis lyrata |
AtBSMT1 | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
benzoic acid | pH 7.7, 30°C | Arabidopsis thaliana | |
0.3 | - |
benzoic acid | pH 7.7, 30°C | Arabidopsis lyrata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Arabidopsis thaliana |
7 | 8 | - |
Arabidopsis lyrata |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | under normal growing conditions AtBSMT1 promoter is only active in leaves at the base of the trichomes, in the hydathodes, and in the upper part of the sepals of flowers. Strong AtBSMT1 promoter activity is observed around lesions in the leaves caused by thrips of the genus Frankliniella | additional information |
Arabidopsis thaliana | leaf wounding and uprooting causes AtBSMT1 transcripts to accumulate quickly within 2 h after treatment with uprooting being more effective | up |
Arabidopsis thaliana | methyl jasmonate treatment, in which plants are exposed to methyl jasmonate vapors in a closed container, results in slow induction | up |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
benzoic acid | pH 7.7, 30°C | Arabidopsis thaliana | |
9.6 | - |
benzoic acid | pH 7.7, 30°C | Arabidopsis lyrata |