Protein Variants | Comment | Organism |
---|---|---|
additional information | lethal phenotype of a DELTAnep1 deletion, overexpression of RPS19B and also deletions within genes DELTAnop6 and DELTAtma23, respectively, which both encode small, positively charged fungal-specific proteins, suppress the DELTAnep1 growth deficiency. Identification of DELTanep1-specific genetic interactions, polysome profiles and phenotypes, overview. DELTAAutp30 enhances the phenotype of DELTAnep1 and is a multi-copy suppressor | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
strains from BY strain background or isogenic to strain CEN.PK2, gene nep1 | - |
Purification (Comment) | Organism |
---|---|
native enzyme partially from subcellular fractions by sucrose density gradient fractionation | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Emg1 | - |
Saccharomyces cerevisiae |
Nep1 | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | lethal phenotype of a DELTAnep1 deletion, deletions in ribosome quality and functional control genes lead to DELTAnep1 growth deficiency. Except for DELTArps18b, deletions in the identified ribosome biogenesis genes are synthetically lethal with DELTAnep1. The DELTAutp30 deletion itself has no phenotype but it enforces all nep1-1ts mutant phenotypes, utp30 overexpression partially restores the nep1-1ts growth deficiency | Saccharomyces cerevisiae |
metabolism | Nep1 interacting genes correspond to ribosome biogenesis, i.e. RPS18A, RPS18B, RRP8, EFG1, UTP30, to ribosome quality control, i.e. UBP3, BRE5, UBP6, and to ribosome functional control, i.e. DOM34, no-go decay | Saccharomyces cerevisiae |
additional information | genome-wide yeast screen to uncover synthetic interactions of DELTAnep1 | Saccharomyces cerevisiae |
physiological function | the nucleolar essential protein Nep1 is an important trans-acting factor in the 90S preribosome. Nep1 methylates the hypermodified psi1191 base of 18S rRNA and has an additional essential function during ribosome biogenesis, i.e. in 40S subunit synthesis. Utp30 and Nep1 act together during pre-ribosomal complex formation and, along with Rps18, provide the surface for the Rps19 assembly to the 90S pre-ribosome | Saccharomyces cerevisiae |