Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Homo sapiens |
38305 | - |
x * 38305, calculated from amino acid sequence | Homo sapiens |
40000 | - |
x * 40000, SDS-PAGE | Sus scrofa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
phenyl Sepharose column chromatography, DEAE cellulose column chromatography, Poros ion exchange column chromatography, DEAE Sephacelcolumn chromatography, and Sephadex G-75 gel filtration | Homo sapiens |
phenyl Sepharose column chromatography, DEAE cellulose column chromatography, Poros ion exchange column chromatography, DEAE Sephacelcolumn chromatography, and Sephadex G-75 gel filtration | Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000006 | - |
crude extract, at 30°C in 20 mM Tris, 0.5 mM dithiothreitol, pH 7.4 | Sus scrofa |
0.0046 | - |
purified enzyme, at 30°C in 20 mM Tris, 0.5 mM dithiothreitol, pH 7.4 | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity changes are observed upon methylation of dimeric and trimeric phosphatase 2A protein | Homo sapiens | ? | - |
? | |
additional information | no activity changes are observed upon methylation of dimeric and trimeric phosphatase 2A protein | Sus scrofa | ? | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine | the enzyme exclusively methylates the carboxyl group of the C-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (Leu309) | Homo sapiens | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine | the enzyme exclusively methylates the carboxyl group of the C-terminal leucine residue of the catalytic subunit of protein phosphatase 2A (Leu309) | Sus scrofa | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine methyl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Homo sapiens |
? | x * 40000, SDS-PAGE | Sus scrofa |
? | x * 38305, calculated from amino acid sequence | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PP2A LCMT | - |
Homo sapiens |
PP2A LCMT | - |
Sus scrofa |
protein phosphatase 2A leucine carboxyl methyltransferase | - |
Homo sapiens |
protein phosphatase 2A leucine carboxyl methyltransferase | - |
Sus scrofa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Homo sapiens | calculated from amino acid sequence | - |
5.7 |