Cloned (Comment) | Organism |
---|---|
the FLAG-tagged-ABH8 coding region is cloned into the pBABEpuro or pBABEhygro retroviral vector. HeLa S3 cells are infected with retrovirus | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA | Homo sapiens | the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96BT7 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA | the enzyme catalyzes tRNA methylation to generate 5-methylcarboxymethyl uridine at the wobble position of certain tRNAs, a critical anticodon loop modification linked to DNA damage survival | Homo sapiens | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | - |
? | |
S-adenosyl-L-methionine + carboxymethyluridine34 in tRNA | ABH8 catalyzes RNA methylation through its methyltransferase domain. ABH8 MT domain alone exhibited weak methylase activity | Homo sapiens | S-adenosyl-L-homocysteine + 5-(2-methoxy-2-oxoethyl)uridine34 in tRNA | 5-(2-methoxy-2-oxoethyl)uridine i.e. 5-methoxycarboxymethyluridine | ? |
Synonyms | Comment | Organism |
---|---|---|
ABH8 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Organism | Comment | Expression |
---|---|---|
Homo sapiens | DNA-damaging agents induce ABH8 expression in an ATM(central DNA damage kinase)-dependent manner | down |
General Information | Comment | Organism |
---|---|---|
malfunction | ABH8 depletion in human cells reduces endogenous levels of 5-methoxycarboxymethyluridine in RNA and increases cellular sensitivity to DNA-damaging agents | Homo sapiens |
physiological function | required for wobble uridine modification and DNA damage survival | Homo sapiens |