BRENDA - Enzyme Database
show all sequences of 2.1.1.228

Codon-specific translation by m1G37 methylation of tRNA

Hou, Y.; Masuda, I.; Gamper, H.; Front. Genet. 10, 713 (2019) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Engineering
Protein Variants
Commentary
Organism
S88L
mutant trmD harbors a mutation near the AdoMet binding site, the mutation prevents the enzyme from binding to the methyl donor and from performing the Mg2C-dependent methyl transfer. The reported observation supports a model of codon-specific translation in the 5'-leader ORF
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Mg2+
required, cells expressing the native trmD show more than a 6fold activation of transcription upon switching from high to low Mg2+ media, whereas cells expressing a S88L mutant trmD show less than a 2fold activation. For cells expressing the native trmD, the level of Mg2+ modulates the level of TrmD-dependent m1G37-tRNA synthesis, which in turn modulates the speed of ribosomal translation of m1G37-dependent codons in the 5'-leader ORF. At high Mg2+, TrmD is active and the abundantly synthesized m1G37-tRNA facilitates ribosomal translation through the 5'-leader ORF
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Saccharomyces cerevisiae ATCC 204508
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
P0A873
-
-
Saccharomyces cerevisiae
P38793
-
-
Saccharomyces cerevisiae ATCC 204508
P38793
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
P36245
-
-
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
P36245
-
-
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
P36245
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Saccharomyces cerevisiae ATCC 204508
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)
-
756774
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
TrmD
-
Escherichia coli
TrmD
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
S-adenosyl-L-methionine
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
S-adenosyl-L-methionine
-
Saccharomyces cerevisiae
S-adenosyl-L-methionine
-
Salmonella enterica subsp. enterica serovar Typhimurium
Engineering (protein specific)
Protein Variants
Commentary
Organism
S88L
mutant trmD harbors a mutation near the AdoMet binding site, the mutation prevents the enzyme from binding to the methyl donor and from performing the Mg2C-dependent methyl transfer. The reported observation supports a model of codon-specific translation in the 5'-leader ORF
Salmonella enterica subsp. enterica serovar Typhimurium
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Escherichia coli
Mg2+
required, cells expressing the native trmD show more than a 6fold activation of transcription upon switching from high to low Mg2+ media, whereas cells expressing a S88L mutant trmD show less than a 2fold activation. For cells expressing the native trmD, the level of Mg2+ modulates the level of TrmD-dependent m1G37-tRNA synthesis, which in turn modulates the speed of ribosomal translation of m1G37-dependent codons in the 5'-leader ORF. At high Mg2+, TrmD is active and the abundantly synthesized m1G37-tRNA facilitates ribosomal translation through the 5'-leader ORF
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Saccharomyces cerevisiae
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Saccharomyces cerevisiae ATCC 204508
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Saccharomyces cerevisiae
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756774
Saccharomyces cerevisiae ATCC 204508
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAPro(UGG)
-
756774
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAPro(UGG)
-
-
-
?
Expression
Organism
Commentary
Expression
Salmonella enterica subsp. enterica serovar Typhimurium
cells expressing the native trmD show more than a 6fold activation of transcription upon switching from high to low Mg2+ media, whereas cells expressing a S88L mutant trmD show less than a 2fold activation. For cells expressing the native trmD, the level of Mg2+ modulates the level of TrmD-dependent m1G37-tRNA synthesis, which in turn modulates the speed of ribosomal translation of m1G37-dependent codons in the 5'-leader ORF. At high Mg2+, TrmD is active and the abundantly synthesized m1G37-tRNA facilitates ribosomal translation through the 5'-leader ORF
up
General Information
General Information
Commentary
Organism
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Escherichia coli
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Saccharomyces cerevisiae
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Salmonella enterica subsp. enterica serovar Typhimurium
malfunction
Lack of m1G37 promotes the tRNA to make +1-frameshifts in a fast mechanism during tRNA translocation from the A- to the P-site on the ribosome, and also in a much slower mechanism during tRNA stalling on the P-site next to an empty A-site
Escherichia coli
additional information
codon-specific translation in Mg2+ homeostasis, overview. Mg2+ homeostasis in Salmonella is maintained by the membrane-bound two-component system PhoPQ sensing of the external low Mg2+, which activates transcription of the major transporter gene mgtA. Transcription of mgtA is determined by ribosomal translation of the 5'-leader ORF, which contains several m1G37-dependent Pro codons
Salmonella enterica subsp. enterica serovar Typhimurium
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, Trm5 is required for growth in the single-cell eukaryote Saccharomyces cerevisiae, where it provides the important role of preventing mis-charging of tRNA
Saccharomyces cerevisiae
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, TrmD is required for growth in several bacterial species, including Escherichia coli and Salmonella
Escherichia coli
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, TrmD is required for growth in several bacterial species, including Escherichia coli and Salmonella
Salmonella enterica subsp. enterica serovar Typhimurium
General Information (protein specific)
General Information
Commentary
Organism
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Escherichia coli
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Saccharomyces cerevisiae
evolution
in the bacterial domain, the biosynthesis of m1G37 is catalyzed by the tRNA methyltransferase TrmD, whereas in the eukaryotic and archaeal domains, it is catalyzed by Trm5. While both TrmD and Trm5 perform the same methyl transfer reaction, using S-adenosyl methionine (AdoMet) as the methyl donor, they are fundamentally different in structure, where TrmD is a member of the SpoU-TrmD family and Trm5 is a member of the Rossmann-fold family. TrmD and Trm5 also differ in virtually all aspects of the reaction mechanism
Salmonella enterica subsp. enterica serovar Typhimurium
malfunction
Lack of m1G37 promotes the tRNA to make +1-frameshifts in a fast mechanism during tRNA translocation from the A- to the P-site on the ribosome, and also in a much slower mechanism during tRNA stalling on the P-site next to an empty A-site
Escherichia coli
additional information
codon-specific translation in Mg2+ homeostasis, overview. Mg2+ homeostasis in Salmonella is maintained by the membrane-bound two-component system PhoPQ sensing of the external low Mg2+, which activates transcription of the major transporter gene mgtA. Transcription of mgtA is determined by ribosomal translation of the 5'-leader ORF, which contains several m1G37-dependent Pro codons
Salmonella enterica subsp. enterica serovar Typhimurium
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, Trm5 is required for growth in the single-cell eukaryote Saccharomyces cerevisiae, where it provides the important role of preventing mis-charging of tRNA
Saccharomyces cerevisiae
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, TrmD is required for growth in several bacterial species, including Escherichia coli and Salmonella
Escherichia coli
physiological function
the N1-methylation of G37 on the 3'-side of the tRNA anticodon, generating m1G37, which as a single methylated nucleobase is not only essential for life but is also conserved in evolution present in all three domains of life. Codon-specific translation by m1G37 methylation of tRNA, mechanism, overview. Maintenance of protein synthesis reading frame by m1G37-tRNA. The maintenance of protein synthesis reading frame in normal cellular conditions is achieved with unexpectedly high fidelity. Due to the dependence on m1G37 for cell survival, TrmD is required for growth in several bacterial species, including Escherichia coli and Salmonella
Salmonella enterica subsp. enterica serovar Typhimurium
Expression (protein specific)
Organism
Commentary
Expression
Salmonella enterica subsp. enterica serovar Typhimurium
cells expressing the native trmD show more than a 6fold activation of transcription upon switching from high to low Mg2+ media, whereas cells expressing a S88L mutant trmD show less than a 2fold activation. For cells expressing the native trmD, the level of Mg2+ modulates the level of TrmD-dependent m1G37-tRNA synthesis, which in turn modulates the speed of ribosomal translation of m1G37-dependent codons in the 5'-leader ORF. At high Mg2+, TrmD is active and the abundantly synthesized m1G37-tRNA facilitates ribosomal translation through the 5'-leader ORF
up
Other publictions for EC 2.1.1.228
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755697
Zhong
Targeting the bacterial epitr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
ACS Infect. Dis.
5
326-335
2019
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1
1
-
-
-
63
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
6
-
3
-
-
1
2
-
-
-
1
-
-
63
-
1
1
1
-
-
-
63
63
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
1
2
-
-
-
-
-
1
1
-
2
2
756152
Li
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Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
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13
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1
1
-
-
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1
-
-
-
-
2
-
7
-
-
1
-
-
1
-
-
2
2
2
-
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
2
-
-
-
-
1
-
-
-
-
2
2
-
-
-
756153
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Biomol. NMR Assign.
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-
-
1
-
-
-
2
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
756774
Hou
Codon-specific translation by ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720, Saccharomyces cerevisiae ATCC 204508
Front. Genet.
10
713
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-
-
-
-
1
-
-
-
-
2
-
7
-
8
-
-
-
-
-
-
-
-
7
-
4
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
8
8
1
-
-
757408
Whitehouse
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1
1
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50
-
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-
1
-
6
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
-
50
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
757409
Zhong
Thienopyrimidinone derivative ...
Staphylococcus aureus, Mycobacterium tuberculosis, Pseudomonas aeruginosa
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7788-7805
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-
-
3
2
-
3
42
3
-
2
-
3
-
5
-
-
-
-
-
-
-
-
3
-
12
1
-
-
-
1
-
-
3
-
-
26
-
-
3
3
2
-
3
26
42
-
3
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
757863
Jin
AtTrm5a catalyses 1-methylgua ...
Arabidopsis thaliana
Nucleic Acids Res.
47
883-898
2019
-
-
1
-
1
-
-
-
1
1
-
2
-
8
-
-
1
-
-
2
-
-
2
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
758329
Jaroensuk
Crystal structure and catalyt ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
RNA
25
1481-1496
2019
-
1
1
1
1
-
1
3
-
2
-
2
-
10
-
-
1
-
-
-
-
-
14
2
5
1
-
-
2
1
-
-
1
3
-
-
-
1
1
1
1
1
-
-
1
3
3
-
2
-
2
-
-
-
1
-
-
-
-
14
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
757201
Zhou
A hypertension-associated mit ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Methanocaldococcus jannaschii JCM 10045
J. Biol. Chem.
293
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2018
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1
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-
12
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5
-
-
-
-
-
-
-
1
-
-
-
-
-
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1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
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1
1
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-
755960
Wu
The crystal structure of the ...
Pyrococcus abyssi
Biochem. Biophys. Res. Commun.
493
240-245
2017
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-
1
1
1
-
-
-
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1
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9
-
-
1
-
-
-
-
-
2
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
756161
Goto-Ito
Trm5 and TrmD two enzymes fr ...
Escherichia coli, Haemophilus influenzae, Methanocaldococcus jannaschii, Pyrococcus abyssi, Pyrococcus abyssi Orsay, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
Biomolecules
7
32
2017
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-
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1
-
-
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4
-
-
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16
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36
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-
-
-
-
-
-
-
30
4
10
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
1
-
-
-
-
-
4
-
-
-
16
-
-
-
-
-
-
-
-
30
4
-
-
-
-
-
-
-
-
-
14
14
-
-
-
756163
Hori
Transfer RNA methyltransferas ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli
Biomolecules
7
E23
2017
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
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6
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
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-
3
-
-
-
-
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
7
7
-
-
-
756631
Hou
TrmD A methyl transferase fo ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Enzymes
41
89-115
2017
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4
4
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-
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16
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12
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8
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-
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12
8
6
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4
-
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-
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4
4
4
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-
-
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16
-
12
-
-
-
-
-
-
-
-
12
8
-
-
-
-
-
-
-
-
-
16
16
-
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-
758348
Wang
Structural insight into the m ...
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Sci. Adv.
3
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2017
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1
2
7
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2
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4
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6
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1
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-
-
7
1
6
2
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-
-
2
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2
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-
-
-
-
1
2
2
7
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
7
1
2
-
-
-
2
-
-
-
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4
4
-
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757790
Christian
Methyl transfer by substrate ...
Haemophilus influenzae
Nat. Struct. Mol. Biol.
23
941-948
2016
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1
-
-
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1
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1
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2
1
2
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1
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1
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1
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1
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2
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-
-
-
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2
1
-
-
-
-
-
-
-
-
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4
4
-
-
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758324
Urbonavicius
Evolution of tRNAPhe imG2 met ...
Nanoarchaeum equitans, Pyrococcus abyssi, Pyrococcus abyssi Orsay
RNA
22
1871-1883
2016
-
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2
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7
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-
-
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2
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11
-
4
-
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2
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19
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14
2
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-
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2
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2
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2
2
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7
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2
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11
-
-
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2
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-
-
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19
-
2
-
-
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2
-
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8
8
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758376
Wang
Crystal structures of the bif ...
Pyrococcus abyssi
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6
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2016
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1
1
2
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1
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9
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1
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3
2
5
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1
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1
1
1
2
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1
-
2
-
-
-
1
-
-
-
-
3
2
-
-
-
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-
-
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4
4
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735421
Powell
TRMT5 mutations cause a defect ...
Homo sapiens
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97
319-328
2015
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1
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3
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1
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1
1
2
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1
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1
1
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3
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1
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1
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-
-
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-
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1
1
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-
-
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-
-
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4
4
-
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737174
Ito
Structural basis for methyl-do ...
Haemophilus influenzae, Thermotoga maritima, Haemophilus influenzae DSM 11121
Proc. Natl. Acad. Sci. USA
112
E4197-E4205
2015
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2
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4
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2
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17
2
2
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1
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2
2
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12
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3
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17
2
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10
1
-
-
-
-
4
4
-
10
10
757582
Hou
Kinetic analysis of tRNA meth ...
Escherichia coli, Haemophilus influenzae, Homo sapiens, Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
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91-116
2015
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28
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26
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13
4
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4
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4
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4
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4
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4
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13
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26
-
4
-
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4
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12
12
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729858
Kawamura
Transfer RNA methyltransferase ...
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
-
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1
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-
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9
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1
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1
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2
1
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1
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-
1
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-
1
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-
-
-
1
-
1
-
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1
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735927
Sakaguchi
A divalent metal ion-dependent ...
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Chem. Biol.
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2014
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1
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1
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4
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1
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1
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1
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2
1
1
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-
1
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1
-
1
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1
-
4
-
1
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2
1
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2
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736428
Masuda
The temperature sensitivity of ...
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288
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1
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1
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1
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3
1
2
1
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6
1
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1
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1
1
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1
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7
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1
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1
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1
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3
1
1
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6
1
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2
2
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6
6
737226
Christian
Conservation of structure and ...
Homo sapiens
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19
1192-1199
2013
-
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1
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9
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3
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-
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1
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12
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1
1
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2
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1
1
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1
1
1
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1
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1
1
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9
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3
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1
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1
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2
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1
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1
1
1
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2
2
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1
1
737228
Paris
The T. brucei TRM5 methyltrans ...
Trypanosoma brucei brucei, Trametes pubescens 927 / 4 GUTat10.1 / TREU927
RNA
19
649-658
2013
-
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1
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1
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2
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2
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5
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1
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12
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1
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1
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1
1
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1
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-
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2
-
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2
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1
-
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-
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12
-
-
-
-
-
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-
-
-
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2
2
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721035
Sakaguchi
Recognition of guanosine by di ...
Escherichia coli, Methanocaldococcus jannaschii
RNA
18
1687-1701
2012
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
2
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10
-
2
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-
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-
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-
-
2
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-
-
2
-
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-
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2
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2
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-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
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2
2
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-
721029
Lahoud
Differentiating analogous tRNA ...
Escherichia coli, Methanocaldococcus jannaschii
RNA
17
1236-1246
2011
-
-
-
-
-
-
24
2
-
-
-
2
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2
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-
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2
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2
2
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2
24
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-
-
-
2
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24
24
2
-
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2
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2
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2
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720240
Christian
Control of catalytic cycle by ...
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204-217
2010
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-
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2
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-
-
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6
2
2
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3
-
-
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2
-
-
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-
-
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2
-
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-
-
-
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2
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-
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4
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6
2
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3
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721026
Christian
Mechanism of N-methylation by ...
Methanocaldococcus jannaschii
RNA
16
2484-2492
2010
-
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1
18
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1
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1
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1
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1
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1
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3
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2
1
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1
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1
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1
1
18
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1
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1
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1
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3
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1
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1
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701123
Goto-Ito
Crystal structure of archaeal ...
Methanocaldococcus jannaschii
Proteins
72
1274-1289
2008
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1
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1
1
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11
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1
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2
1
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1
1
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1
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1
-
1
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1
-
1
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1
1
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-
-
-
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1
1
712103
Toyooka
Stabilization of tRNA (mG37) m ...
Aquifex aeolicus
Genes Cells
13
807-816
2008
-
-
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1
-
-
-
-
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1
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1
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1
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1
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1
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1
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1
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687609
Lee
Yeast mitochondrial initiator ...
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1
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1
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1
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1
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2
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1
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Distinct determinants of tRNA ...
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5
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5
2
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2
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5
673850
Takeda
The substrate specificity of t ...
Aquifex aeolicus
Genes Cells
11
1353-1365
2006
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1
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13
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1
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6
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1
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15
-
3
1
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1
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-
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-
1
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