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Literature summary for 2.1.1.192 extracted from
- The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA (2008), RNA, 14, 98-106.
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | Escherichia coli | the enzyme catalyzes the natural post-transcriptional modification of A2503 to m2A in Escherichia coli 23S rRNA | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA | - |
? | |
| 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | the enzyme catalyzes the natural post-transcriptional modification of A2503 to m2A in Escherichia coli 23S rRNA | Escherichia coli | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| methyltransferase YfgB/RlmN | - |
Escherichia coli |
| RlmN | - |
Escherichia coli |
| YfgB | - |
Escherichia coli |
| YfgB/RlmN | - |
Escherichia coli |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | inactivation of the yfgB gene in Escherichia coli leads to the loss of modification at nucleotide A2503 of 23S rRNA. The A2503 modification is restored when YfgB protein is expressed in the yfgB knockout strain. In a co-growth competition experiment, the rlmN knockout mutant shows reduced fitness compared to wild type. The absence of m2A2503 modification produces a subtle but significant effect on cell fitness apparently through its effect on protein synthesis or ribosome assembly. Escherichia coli rlmN knockout strain shows a small but reproducible twofold increased susceptibility to tiamulin, hygromycin A, and sparsomycin compared to the wild-type strain | Escherichia coli |
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Release 2023.1 (January 2023)
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