Cloned (Comment) | Organism |
---|---|
the rsmC gene, cloned into pCA24N vector with a noncleavable N-terminal His6 tag | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal structure of RsmC refined to 2.1 A resolution, reveals two homologous domains tandemly duplicated within a single polypeptide. Characterization of the function of the individual domains and identification of key residues involved in binding of rRNA and S-adenosyl-L-methionine, and in catalysis. It is discovered that one of the domains is important for the folding of the other. RsmC can be regarded as a model system for functional streamlining of domains accompanied by the development of dependencies concerning folding and stability | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1207 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39406 | - |
- |
Purification (Comment) | Organism |
---|---|
purification and refolding of C-RsmC from inclusion bodies | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1207 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RsmC | - |
Escherichia coli |