Cloned (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53300 | - |
x * 53300, SDS-PAGE | Arabidopsis thaliana |
53500 | - |
x * 53500, SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Arabidopsis thaliana | the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | O80562 | - |
- |
Arabidopsis thaliana | Q0WP12 | - |
- |
Arabidopsis thaliana | Q6AWU6 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000058 | - |
production of methyl iodide | Arabidopsis thaliana |
0.00009 | - |
production of methyl iodide | Arabidopsis thaliana |
205 | - |
production of methyl iodide | Arabidopsis thaliana |
Storage Stability | Organism |
---|---|
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 40% of the iodide methyltransferase activity | Arabidopsis thaliana |
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 60% of the iodide methyltransferase activity | Arabidopsis thaliana |
-20°C, purified protein stored in a buffer (140 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, 1 mM DTT, 30% glycerol), after 15 days, the recombinant protein AtHOL1 retains 90% of the iodide methyltransferase activity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the activation of AtHOL1, AtHOL2 and AtHOL3 genes contributes to the methyl halide emissions from Arabidopsis | Arabidopsis thaliana | ? | - |
? | |
S-adenosyl-L-methionine + chloride | - |
Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl chloride | - |
? | |
S-adenosyl-L-methionine + iodide | recombinant protein methylates iodide with greater efficiency than chloride | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl iodide | - |
? | |
S-adenosyl-L-methionine + iodide | recombinant proteins methylate iodide with greater efficiency than chloride | Arabidopsis thaliana | S-adenosyl-L-homocysteine + methyl iodide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53500, SDS-PAGE | Arabidopsis thaliana |
? | x * 53300, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
AtHOL1 | - |
Arabidopsis thaliana |
AtHOL2 | - |
Arabidopsis thaliana |
AtHOL3 | - |
Arabidopsis thaliana |