Application | Comment | Organism |
---|---|---|
additional information | autoregulates its own translation, RNA stem-loop structure acts as an inhibitory regulator of translation by preventing the binding of its Shine-Dalgarno-like sequence by positioning it in the stem region, addition of Thy1 into the in vitro translation system also inhibits translation | Pyrococcus furiosus |
Cloned (Comment) | Organism |
---|---|
overexpression of the recombinant C-terminal His6-tagged protein in Escherichia coli strain BL21(DE3) | Pyrococcus furiosus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 0.5 mM enhances the activity | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, sequence analysis, x * 29000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | Q8U3C9 | DSM3638 | - |
Purification (Comment) | Organism |
---|---|
to near homogeneity, by heat treatment at 85°C for 15 min, followed by Ni2+ column chromatography | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | possesses RNA-binding activity, binds to the stemloop RNA structure | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, sequence analysis, x * 29000, SDS-PAGE | Pyrococcus furiosus |
Synonyms | Comment | Organism |
---|---|---|
flavin-dependent thymidylate synthase | - |
Pyrococcus furiosus |
Thy1 | - |
Pyrococcus furiosus |
thymidylate synthase 1 | - |
Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
optimum RNA-binding activity | Pyrococcus furiosus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
no RNA-binding activity at 95°C | Pyrococcus furiosus |