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Literature summary for 2.1.1.103 extracted from
- Conformational changes in the di-domain structure of Arabidopsis phosphoethanolamine methyltransferase leads to active-site formation (2017), J. Biol. Chem., 292, 21690-21702 .
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| structure in complex with S-adenosyl-L-methionine | Arabidopsis thaliana |
| structure in complex with S-adenosyl-L-methionine, to 1.5 A resolution | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.069 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana |  |
| 0.0897 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.093 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.156 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.201 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.212 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.215 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.249 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.278 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.284 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.308 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.408 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.507 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.577 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.653 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q944H0 | - |
- |
| Arabidopsis thaliana | Q9C6B9 | - |
- |
| Arabidopsis thaliana | Q9FR44 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ethanolamine phosphate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | N-methylethanolamine phosphate + S-adenosyl-L-homocysteine | - |
? | |
| N,N-dimethylethanolamine phosphate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | choline phosphate + S-adenosyl-L-homocysteine | - |
? | |
| N-methylethanolamine phosphate + S-adenosyl-L-methionine | - |
Arabidopsis thaliana | N,N-dimethylethanolamine phosphate + S-adenosyl-L-homocysteine | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 56100, calculated from sequence | Arabidopsis thaliana |
| ? | x * 57800, calculated from sequence | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.04 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.04 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.06 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.09 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.13 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.14 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.17 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.21 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.21 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.21 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.22 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.27 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.27 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.27 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | isoform NMT1 is essential for normal growth and salt tolerance. The NMT1 mutant displays a short root phenotype under normal growth conditions and hypersensitivity, as determined by root length, under salt-stress conditions | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.161 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.209 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.266 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.305 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.367 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.408 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.433 | - |
N,N-dimethylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.489 | - |
N-methylethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.658 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.767 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.866 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 0.897 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.006 | - |
S-adenosyl-L-methionine | cosubstrate N,N-dimethylethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.314 | - |
Ethanolamine phosphate | pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
| 1.453 | - |
S-adenosyl-L-methionine | cosubstrate ethanolamine phosphate, pH 8.0, temperature not specified in the publication | Arabidopsis thaliana | |
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