Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K10A | about 40% of wild-type activity | Rattus norvegicus |
K7A | slight decrease in activity | Rattus norvegicus |
K84A | about 45% of wild-type activity | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | mainly cytoplasmic localization in liver | Rattus norvegicus | 5737 | - |
nucleus | in most tissues, low expression of BHMT coincides with a preferential nuclear localization of the protein | Rattus norvegicus | 5634 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | O09171 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | low expression level | Rattus norvegicus | - |
cerebellum | low expression level | Rattus norvegicus | - |
kidney | high expression level | Rattus norvegicus | - |
liver | highest expression level detected | Rattus norvegicus | - |
lung | low expression level | Rattus norvegicus | - |
skeletal muscle | low expression level | Rattus norvegicus | - |
testis | low expression level | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0044 | - |
mutant K10A, pH 7.5, 37°C | Rattus norvegicus |
0.0047 | - |
mutant K8A, pH 7.5, 37°C | Rattus norvegicus |
0.0109 | - |
mutant K7A, pH 7.5, 37°C | Rattus norvegicus |
0.01215 | - |
wild-type, pH 7.5, 37°C | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
betaine + L-homocysteine | - |
Rattus norvegicus | dimethylglycine + L-methionine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
BHMT | - |
Rattus norvegicus |
General Information | Comment | Organism |
---|---|---|
physiological function | oxidative stress associated with D-galactosamine (Gal) or buthionine sulfoximine (BSO) treatments induces BHMT translocation from cytoplasm to nucleus, which is prevented by administration of N-acetylcysteine and glutathione ethyl ester, respectively. The hepatic nuclear accumulation induced by Gal associates with reduced nuclear BHMT activity and a trend towards increased protein homocysteinylation | Rattus norvegicus |