Crystallization (Comment) | Organism |
---|---|
structure in complex with glutathione, to 2.5 A resolution. Isoform GHR1 adopts a typical glutathionyl-hydroquinone reductase fold, with a dimerization interface comparable to that of the bacterial and fungal counterparts | Populus trichocarpa |
Protein Variants | Comment | Organism |
---|---|---|
C49A | mutation of catalytic cysteine, loss of activity | Populus trichocarpa |
C49S | mutation of catalytic cysteine, loss of activity | Populus trichocarpa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
2-(glutathione-S-yl)-quercetin | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
0.003 | - |
2-(glutathione-S-yl)-menadiol | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
0.072 | - |
(glutathione-S-yl)-2-chloro-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
0.0926 | - |
2-(glutathione-S-yl)-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
0.27 | - |
2-(glutathione-S-yl)-menadione | wild-type, pH 8.0, 20°C | Populus trichocarpa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
plastid | - |
Populus trichocarpa | 9536 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Populus trichocarpa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | primarily expressed in reproductive organs | Populus trichocarpa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + (glutathione-S-yl)-2-chloro-1,4-benzoquinone | - |
Populus trichocarpa | glutathione disulfide + 2-chloro-1,4-benzoquinone | - |
? | |
glutathione + 2'-(glutathione-S-yl)-quercetin | - |
Populus trichocarpa | glutathione disulfide + quercetin | - |
? | |
glutathione + 2-(glutathione-S-yl)-1,4-benzoquinone | - |
Populus trichocarpa | glutathione disulfide + 1,4-benzoquinone | - |
? | |
glutathione + 2-(glutathione-S-yl)-menadiol | - |
Populus trichocarpa | glutathione disulfide + menadiol | - |
? | |
glutathione + 2-(glutathione-S-yl)-menadione | - |
Populus trichocarpa | glutathione disulfide + menadione | - |
? | |
glutathione + 2-(glutathione-S-yl)-quercetin | - |
Populus trichocarpa | glutathione disulfide + quercetin | - |
r | |
additional information | isoform GHR1 catalyzes the deglutathionylation of both reduced and oxidized glutathionylated quinones, but the enzyme is more catalytically efficient with the reduced forms | Populus trichocarpa | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GHR1 | - |
Populus trichocarpa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
2-(glutathione-S-yl)-quercetin | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
1.62 | - |
2-(glutathione-S-yl)-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
4.75 | - |
2-(glutathione-S-yl)-menadione | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
7.96 | - |
(glutathione-S-yl)-2-chloro-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
9.42 | - |
2-(glutathione-S-yl)-menadiol | wild-type, pH 8.0, 20°C | Populus trichocarpa |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.95 | - |
S-glutathionylquercetin | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
17.4 | - |
2-(glutathione-S-yl)-menadione | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
17.5 | - |
2-(glutathione-S-yl)-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
110.6 | - |
(glutathione-S-yl)-2-chloro-1,4-benzoquinone | wild-type, pH 8.0, 20°C | Populus trichocarpa | |
3215 | - |
2-(glutathione-S-yl)-menadiol | wild-type, pH 8.0, 20°C | Populus trichocarpa |