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Literature summary for 1.8.4.11 extracted from

  • Kuschel, L.; Hansel, A.; Schonherr, R.; Weissbach, H.; Brot, N.; Hoshi, T.; Heinemann, S.H.
    Molecular cloning and functional expression of a human peptide methionine sulfoxide reductase (hMsrA) (1999), FEBS Lett., 456, 17-21.
    View publication on PubMed
Search for more literature for 1.8.4.11

Cloned(Commentary)

Cloned (Comment) Organism
gene msrA, DNA and amino acid sequence determination and analysis, expression analysis, co-expression of A-type potassium channel ShC/B and MsrA in Xenopus laevis oocytes significantly accelerating the inactivation of the channel protein, functional expression of MsrA as GST-fusion protein in Escherichia coli strain BL21 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptide-L-methionine-(S)-S-oxide + thioredoxin Homo sapiens substrate is oxidized A-type potassium channel ShC/B whose activity strongly depends on the oxidative state of a methionine residue in the N-terminal part of the polypeptide peptide-L-methionine + thioredoxin disulfide + H2O
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 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
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-
-

Source Tissue

Source Tissue Comment Organism Textmining
bone marrow high MsrA expression level Homo sapiens
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cerebellum high MsrA expression level Homo sapiens
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heart ventricle, high MsrA expression level Homo sapiens
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hippocampus high MsrA expression level Homo sapiens
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kidney high MsrA expression level Homo sapiens
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liver high MsrA expression level, especially in fetal liver Homo sapiens
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additional information tissue expression analysis of MsrA, wide tissue distribution, no expression in leukemia and lymphoma cell lines Homo sapiens
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-methionine-(S)-S-oxide + DTT stereospecific reduction Homo sapiens N-acetyl-L-methionine + DTT disulfide + H2O
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 ?
N-acetyl-L-methionine-(S)-S-oxide + thioredoxin stereospecific reduction Homo sapiens N-acetyl-L-methionine + thioredoxin disulfide + H2O
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 ?
peptide-L-methionine-(S)-S-oxide + thioredoxin substrate is oxidized A-type potassium channel ShC/B whose activity strongly depends on the oxidative state of a methionine residue in the N-terminal part of the polypeptide Homo sapiens peptide-L-methionine + thioredoxin disulfide + H2O
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 ?

Synonyms

Synonyms Comment Organism
MsrA
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 Homo sapiens
peptide methionine sulfoxide reductase
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 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
dithiothreitol
-
 Homo sapiens
thioredoxin
-
 Homo sapiens