Cloned (Comment) | Organism |
---|---|
gene msrA, expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | Escherichia coli | enzyme is involved in repairing of oxidized methionine residues in proteins | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | Escherichia coli | physiological role | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene msrA, enzyme is organized in a fusion protein together with MsrB, EC 1.8.4.B1 | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.33 | - |
in vitro, substrate free L-methionine (R)-sulfoxide | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine (S)-sulfoxide + thioredoxin | enzyme is involved in repairing of oxidized methionine residues in proteins | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
L-methionine (S)-sulfoxide + thioredoxin | MsrA is specific for the S-form of the substrate | Escherichia coli | L-methionine + thioredoxin disulfide + H2O | - |
? | |
additional information | physiological role | Escherichia coli | ? | - |
? | |
oxidized calmodulin + thioredoxin | enzyme reduces L-methionine (S)-sulfoxide of the protein substrate | Escherichia coli | partially reduced calmodulin + thioredoxin disulfide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methionine sulfoxide reductase | - |
Escherichia coli |
MsrA | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thioredoxin | - |
Escherichia coli |