Protein Variants | Comment | Organism |
---|---|---|
Y343F | in the mutant enzyme using cytochrome c as electron acceptor, turnover number is somewhat impaired, 34% of the wild-type activity at pH 8.5. The KM-value for the mutant enzyme shows a 5fold increase over wild-type. Reduction of the molybdenum center of the Y343 F variant by sulfite is more significantly impaired at high pH than at low pH | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00129 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00162 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00272 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00311 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00339 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00352 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00362 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.00367 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.00414 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00423 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00435 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00459 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00503 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00612 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.00635 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00728 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.00825 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00851 | - |
sulfite | 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00859 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00908 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00924 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00947 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00959 | - |
sulfite | 25°C, pH 8.75, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.00963 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.00992 | - |
sulfite | 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.011 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.0158 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.0221 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.026 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.0319 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.0536 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
0.0557 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.0671 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
0.147 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
0.59 | 1 | sulfite | 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
1.54 | - |
sulfite | 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sulfite + O2 + H2O | - |
Homo sapiens | sulfate + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.06 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
3.11 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
3.26 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
4.14 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
4.59 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
6.35 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
7.17 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
8.21 | - |
sulfite | 25°C, pH 10.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
8.51 | - |
sulfite | 25°C, pH 10.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
8.59 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
8.72 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.08 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.2 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.24 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.47 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.63 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.92 | - |
sulfite | 25°C, pH 9.75, 20 mM buffer, mutant enzyme Y343F | Homo sapiens | |
9.99 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
10.5 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, mutant enzyme Y343F | Homo sapiens | |
12.1 | - |
sulfite | 25°C, pH 7.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
13.2 | - |
sulfite | 25°C, pH 6.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
17.2 | - |
sulfite | 25°C, pH 7.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
17.7 | - |
sulfite | 25°C, pH 6.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
23.6 | - |
sulfite | 25°C, pH 8.75, 20 mM buffer, wild-type enzyme | Homo sapiens | |
24.2 | - |
sulfite | 25°C, pH 7.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
24.6 | - |
sulfite | 25°C, pH 9.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
24.7 | - |
sulfite | 25°C, pH 7.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
24.8 | - |
sulfite | 25°C, pH 8.25, 20 mM buffer, wild-type enzyme | Homo sapiens | |
25 | - |
sulfite | 25°C, pH 8.0, 100 mM buffer, wild-type enzyme | Homo sapiens | |
25.7 | - |
sulfite | 25°C, pH 9.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
25.9 | - |
sulfite | 25°C, pH 8.0, 20 mM buffer, wild-type enzyme | Homo sapiens | |
26.3 | - |
sulfite | 25°C, pH 9.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
26.9 | - |
sulfite | 25°C, pH 8.5, 100 mM buffer, wild-type enzyme | Homo sapiens | |
26.9 | - |
sulfite | 25°C, pH 8.5, 20 mM buffer, wild-type enzyme | Homo sapiens | |
27 | - |
sulfite | 25°C, pH 8.25, 100 mM buffer, wild-type enzyme | Homo sapiens | |
28.1 | - |
sulfite | 25°C, pH 9.0, 100 mM buffer, wild-type enzyme | Homo sapiens |