Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteine | - |
Triticum aestivum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a His-tagged protein | Triticum aestivum |
expression of wild-type as His-tagged protein and mutant C53S in Escherichia coli, DNA sequence analysis | Triticum aestivum |
Protein Variants | Comment | Organism |
---|---|---|
C53S | site-directed mutagenesis, no activity since the redox cycle system is abolished | Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NADPH | - |
Triticum aestivum | |
0.0004 | - |
NADPH | wild-type | Triticum aestivum | |
0.00076 | - |
thioredoxin | assay with DTNB instead of NADPH | Triticum aestivum | |
0.0076 | - |
thioredoxin disulfide h | - |
Triticum aestivum | |
0.036 | - |
thioredoxin | wild-type, thioredoxin from E. coli | Triticum aestivum | |
0.6 | - |
NADH | - |
Triticum aestivum | |
0.6 | - |
NADH | wild-type | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
x * 35000, SDS-PAGE | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | Q8VX47 | - |
- |
Triticum aestivum | Q8VX47 | cv. Chineses Spring | - |
Purification (Comment) | Organism |
---|---|
recombinant, wild-type and mutant C53S | Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Triticum aestivum | - |
leaf | from mature plants, high accumulation | Triticum aestivum | - |
root | from 2-4-day-old seedlings, high accumulation | Triticum aestivum | - |
seed | aleurone layer, scutellum, starchy endosperm | Triticum aestivum | - |
seed | low accumulation | Triticum aestivum | - |
seedling | - |
Triticum aestivum | - |
seedling | root and shoot | Triticum aestivum | - |
shoot | from 2-4-day-old seedlings, high accumulation | Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | redox system for electron transfer in the complex of apoenzyme, FAD and thioredoxin with NADPH or other electron acceptors | Triticum aestivum | ? | - |
? | |
thioredoxin + NADP+ | coupled assay with DTNB | Triticum aestivum | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | coupled assay, measurement of NADPH oxidation in presence of insulin and thioredoxin | Triticum aestivum | thioredoxin disulfide + NADPH | - |
? | |
thioredoxin + NADP+ | in presence of NADPH, coupled assay | Triticum aestivum | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin + NADP+ | i.e. DTNB | Triticum aestivum | thioredoxin disulfide + NADPH + H+ | - |
? | |
thioredoxin disulfide h + NADH | - |
Triticum aestivum | thioredoxin + NAD+ | - |
? | |
thioredoxin disulfide h + NADPH | - |
Triticum aestivum | thioredoxin + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 35000, SDS-PAGE | Triticum aestivum |
Synonyms | Comment | Organism |
---|---|---|
NTR | - |
Triticum aestivum |
thioredoxin h reductase | - |
Triticum aestivum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Triticum aestivum | |
NADPH | specific for | Triticum aestivum |