Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | GRase-1 is moderately sensitive to inhibition by Co2+ | Phaeodactylum tricornutum | |
Cu2+ | GRase-1 is very sensitive to inhibition by Cu2+ | Phaeodactylum tricornutum | |
glutathione | GSH functions as an inhibitor at relatively high concentrations (41 mM), and also only in the lower substrate concentration range | Phaeodactylum tricornutum | |
NADP+ | competitive product inhibition regarding NADPH and non-competitive product inhibition regarding glutathione disulfide, however, NADP+ (up to 1m M) is not inhibitor when assays are performed at 1 mM glutathione disulfide and 300 mM NADPH | Phaeodactylum tricornutum | |
Ni2+ | - |
Phaeodactylum tricornutum | |
Zn2+ | GRase-1 is very sensitive to inhibition by Zn2+ | Phaeodactylum tricornutum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
NADPH | at 30°C and pH 7.5 | Phaeodactylum tricornutum | |
0.06 | - |
glutathione disulfide | at 30°C and pH 7.5 | Phaeodactylum tricornutum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
composed of two major proteins of 58000 and 40000 Da, SDS-PAGE | Phaeodactylum tricornutum |
53470 | - |
subunit, calculated from amino acid sequence | Phaeodactylum tricornutum |
58000 | - |
composed of two major proteins of 58000 and 40000 Da, SDS-PAGE | Phaeodactylum tricornutum |
59000 | - |
2 * 59000, native PAGE | Phaeodactylum tricornutum |
118000 | - |
native molecular mass, native PAGE | Phaeodactylum tricornutum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phaeodactylum tricornutum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, Blue-A Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography | Phaeodactylum tricornutum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.46 | - |
enzyme from soluble extract, at 30°C and pH 7.5 | Phaeodactylum tricornutum |
73.3 | - |
after 161fold purification, at 30°C and pH 7.5 | Phaeodactylum tricornutum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione disulfide + NADPH + H+ | - |
Phaeodactylum tricornutum | glutathione + NADP+ | using levels up to 300 mM NADP+ and 1 mM glutathione, the enzyme does not catalyse the reverse reaction, indicating that its physiological function is the reduction of GSSG or GSNO but not the opposite | ir | |
additional information | the enzyme is not effective to reduce cystine, lipoamide, nor trypanothione disulfide | Phaeodactylum tricornutum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59000, native PAGE | Phaeodactylum tricornutum |
Synonyms | Comment | Organism |
---|---|---|
glutathione reductase | - |
Phaeodactylum tricornutum |
glutathione:NADP+ oxidoreductase | - |
Phaeodactylum tricornutum |
GRase-1 | - |
Phaeodactylum tricornutum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Phaeodactylum tricornutum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | the abrupt decrease in activity observed at temperatures higher than 35°C, can be associated with loss of enzyme stability beyond 30°C | Phaeodactylum tricornutum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Phaeodactylum tricornutum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | in 100 mM Tris-HCl buffer | Phaeodactylum tricornutum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | the enzyme remains stable in 100 mM Tris-HCl buffer between pH 6.5 and 10.0, the activity remains practically unchanged between pH 6.5-7.5 and decreases significantly only above pH 8.5 | Phaeodactylum tricornutum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | utilizes NADPH but not NADH as electron donor | Phaeodactylum tricornutum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.052 | - |
NADP+ | competitive product inhibition regarding NADPH, at 30°C and pH 7.5 | Phaeodactylum tricornutum | |
1 | - |
NADP+ | non-competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5 | Phaeodactylum tricornutum | |
5 | - |
glutathione | competitive product inhibition regarding glutathione disulfide, at 30°C and pH 7.5 | Phaeodactylum tricornutum | |
10 | - |
glutathione | non-competitive product inhibition regarding NADPH, at 30°C and pH 7.5 | Phaeodactylum tricornutum |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Phaeodactylum tricornutum | calculated from amino acid sequence | - |
5 |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0034 | - |
at 30°C and pH 7.5 | Phaeodactylum tricornutum | Cu2+ | |
0.0042 | - |
at 30°C and pH 7.5 | Phaeodactylum tricornutum | Zn2+ | |
0.036 | - |
at 30°C and pH 7.5 | Phaeodactylum tricornutum | Co2+ | |
0.534 | - |
at 30°C and pH 7.5 | Phaeodactylum tricornutum | Ni2+ |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme's physiological function is the reduction of glutathione disulfide or GSNO but not the opposite | Phaeodactylum tricornutum |