Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | - |
Rhodococcus erythropolis | |
Ni2+ | - |
Rhodococcus erythropolis | |
Zn2+ | - |
Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
4,4'-disulfanediyldibutanoate | at pH 8.0 and 37°C | Rhodococcus erythropolis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal cofactors are required | Rhodococcus erythropolis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4,4'-disulfanediyldibutanoate + NADH + H+ | Rhodococcus erythropolis | the enzyme shows high substrate specificity | 4-mercaptobutyric acid + NAD+ | - |
? | |
4,4'-disulfanediyldibutanoate + NADH + H+ | Rhodococcus erythropolis MI2 | the enzyme shows high substrate specificity | 4-mercaptobutyric acid + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
- |
- |
Rhodococcus erythropolis MI2 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Rhodococcus erythropolis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.36 | - |
at pH 8.0 and 37°C | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4,4'-disulfanediyldibutanoate + NADH + H+ | the enzyme shows high substrate specificity | Rhodococcus erythropolis | 4-mercaptobutyric acid + NAD+ | - |
? | |
4,4'-disulfanediyldibutanoate + NADH + H+ | the enzyme shows high substrate specificity | Rhodococcus erythropolis MI2 | 4-mercaptobutyric acid + NAD+ | - |
? | |
additional information | no activity with NADPH | Rhodococcus erythropolis | ? | - |
? | |
additional information | the enzyme has very low activity with 3,3'-dithiodipropionic acid (8.0%), 3,3'-thiodipropionic acid (7.6%), and 5,5'-dithiobis(2-nitrobenzoic acid) (8.0%) | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with NADPH | Rhodococcus erythropolis MI2 | ? | - |
? | |
additional information | the enzyme has very low activity with 3,3'-dithiodipropionic acid (8.0%), 3,3'-thiodipropionic acid (7.6%), and 5,5'-dithiobis(2-nitrobenzoic acid) (8.0%) | Rhodococcus erythropolis MI2 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Rhodococcus erythropolis |
? | x * 43573, calculated from amino acid sequence | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
NADH:flavin oxidoreductase | - |
Rhodococcus erythropolis |
NOX | - |
Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | after heating the enzyme for 15 min at 85°C, it retains about 48% of its original activity, however, a stable activity could be recorded at 65°C | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
4,4'-disulfanediyldibutanoate | at pH 8.0 and 37°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | - |
Rhodococcus erythropolis | |
NADH | the enzyme is highly specific for NADH | Rhodococcus erythropolis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodococcus erythropolis | calculated from amino acid sequence | - |
8.8 |