Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is dependent on a redox-active cysteine at the active centre | Trypanosoma cruzi |
Crystallization (Comment) | Organism |
---|---|
hanging drop method in presence of 2 M (NH4)2SO4 at 4°C | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | inhibition only of the enzyme with reduced cysteine residues in the catalytic center by alkylation | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km dependent on assay conditions | Trypanosoma cruzi | |
0.005 | 0.007 | NADPH | - |
Trypanosoma cruzi | |
0.022 | 0.275 | glutathionylspermidine disulfide | - |
Trypanosoma cruzi | |
0.045 | - |
trypanothione disulfide | - |
Trypanosoma cruzi | |
0.275 | - |
glutathionylspermidine disulfide | - |
Trypanosoma cruzi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, SDS-PAGE | Trypanosoma cruzi |
100000 | - |
gel filtration | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Trypanosoma cruzi |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
284 | - |
purified enzyme | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathionylspermidine disulfide + NADPH | - |
Trypanosoma cruzi | 2-glutathionylspermidine + NADP+ | - |
r | |
trypanothione disulfide + NADPH | - |
Trypanosoma cruzi | trypanothione + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, SDS-PAGE | Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
237 | - |
trypanothione disulfide | dependent on assay conditions | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Trypanosoma cruzi | |
NADH | low activity with NADH | Trypanosoma cruzi | |
NADPH | enzyme also utilizes NADH, but less efficiently | Trypanosoma cruzi |