KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
hydrazine | pH 7.5, 25°C, with enzyme form HAO-A, that forms only the monocyano derivative of heme P460 | Nitrosomonas europaea | |
0.005 | - |
hydrazine | pH 7.5, 25°C, with enzyme form HAO-B, that forms a mono- and dicyano complex | Nitrosomonas europaea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nitrosomonas europaea | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydrazine + ferricytochrome c | - |
Nitrosomonas europaea | ? + ferrocytochrome c | - |
? | |
hydroxylamine + ferricytochrome c + H2O | - |
Nitrosomonas europaea | nitrite + ferrocytochrome c + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | hypothesis that the enzyme is functionally a dimer in which electrons rapidly equilibrate between the c-hemes of each subunit but not between oligomers | Nitrosomonas europaea |
Synonyms | Comment | Organism |
---|---|---|
HAO | - |
Nitrosomonas europaea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | each subunit of the enzyme contains 7 c-hemes and 1 heme P460. The latter, a c-heme crosslinked from a methylene carbon to the ring of a protein tyrosine, forms part of the active site | Nitrosomonas europaea |