Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0063 | - |
ferrileghemoglobin | recombinant enzyme | Glycine max | |
0.0092 | - |
ferrileghemoglobin | native enzyme | Glycine max | |
0.046 | - |
NADH | lipoamide-dependent NADH oxidation | Glycine max | |
0.716 | - |
Lipoamide | lipoamide-dependent NADH oxidation | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + ferrileghemoglobin | Glycine max | proposed to be involved in maintaining leghemoglobin in an active form in legume nodules | NAD(P)+ + ferroleghemoglobin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, Sepharose 6B, Probond | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.486 | - |
recombinant enzyme, reduction of ferrileghemoglobin | Glycine max |
4.47 | - |
recombinant enzyme, reduction of 2,6-dichloroindophenol | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + ferrileghemoglobin | proposed to be involved in maintaining leghemoglobin in an active form in legume nodules | Glycine max | NAD(P)+ + ferroleghemoglobin | - |
? | |
NADH + lipoamide | recombinant enzyme, lipoamide-dependent NADH oxidation | Glycine max | NAD+ + lipoamide | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.2 | - |
ferrileghemoglobin | recombinant enzyme | Glycine max | |
6.2 | - |
ferrileghemoglobin | native enzyme | Glycine max | |
31 | - |
Lipoamide | recombinant enzyme | Glycine max |