Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Amobarbital | 1 mM, 35% after 0.5-1 h, 80% after 4 h | Glycine max | |
catalase | possibly H2O2 as reaction intermediate | Glycine max | |
iodoacetamide | 1 mM, 95-100% inhibition | Glycine max | |
nicotinate | ferrileghemoglobin-nitrite complex | Glycine max | |
p-hydroxymercuribenzoate | 0.5 mM, slight inhibition | Glycine max | |
Quinacrine | 0.5 mM, 30-40% inhibition | Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0133 | - |
ferrileghemoglobin | - |
Phaseolus vulgaris | |
0.0133 | - |
ferrileghemoglobin | - |
Vigna unguiculata | |
0.088 | - |
ferrileghemoglobin | - |
Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Glycine max |
55000 | - |
2 * 55000, SDS-PAGE | Vigna unguiculata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + ferrileghemoglobin | Glycine max | - |
NAD(P)+ + ferroleghemoglobin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
soybean | - |
Phaseolus vulgaris | - |
bean | - |
Vigna unguiculata | - |
cowpea | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | - |
Phaseolus vulgaris | - |
root nodule | - |
Glycine max | - |
root nodule | - |
Vigna unguiculata | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.215 | - |
reduction of ferrileghemoglobin | Glycine max |
2 | - |
reduction of 2,6-dichlorophenolindophenol | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
NAD(P)H + ferrileghemoglobin | - |
Glycine max | NAD(P)+ + ferroleghemoglobin | - |
? | |
NAD(P)H + ferrileghemoglobin | - |
Vigna unguiculata | NAD(P)+ + ferroleghemoglobin | - |
? | |
NADH + H+ + ferrileghemoglobin | - |
Phaseolus vulgaris | NAD+ + ferroleghemoglobin | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Glycine max |
dimer | 2 * 55000, SDS-PAGE | Vigna unguiculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Phaseolus vulgaris | |
NADH | - |
Glycine max | |
NADH | - |
Vigna unguiculata | |
NADPH | 54-80% of NADH activity | Phaseolus vulgaris | |
NADPH | 54-80% of NADH activity | Glycine max | |
NADPH | 54-80% of NADH activity | Vigna unguiculata |