Activating Compound | Comment | Organism | Structure |
---|---|---|---|
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0041 | - |
4-nitrosoresorcinol-1-monomethyl ether | pH 5.0, in the presence of peroxidase, temperature not specified in the publication | Zea mays | |
0.0062 | - |
4-nitrosoresorcinol-1-monomethyl ether | pH 5.0, in the presence of laccase, temperature not specified in the publication | Zea mays | |
0.0089 | - |
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | pH 5.0, in the presence of peroxidase, temperature not specified in the publication | Zea mays | |
0.0109 | - |
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | pH 5.0, in the presence of laccase, temperature not specified in the publication | Zea mays | |
0.0113 | - |
4-nitrosoresorcinol-1-monomethyl ether | pH 7.0, in the presence of peroxidase, temperature not specified in the publication | Zea mays | |
0.0345 | - |
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | pH 7.0, in the presence of laccase, temperature not specified in the publication | Zea mays | |
0.067 | - |
2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical | pH 5.0, temperature not specified in the publication | Zea mays | |
0.113 | - |
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | pH 7.0, in the presence of peroxidase, temperature not specified in the publication | Zea mays | |
0.192 | - |
4-nitrosoresorcinol-1-monomethyl ether | pH 5.0, temperature not specified in the publication | Zea mays | |
0.25 | - |
hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | Km above 0.25 mM, pH 7.0, temperature not specified in the publication | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
variety Cellux 225 | - |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | laccase and peroxidase catalyze oxidative cleavage of hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one (DIMBOA) to 4-nitrosoresorcinol-1-monomethyl ether (coniferron) which serves as a weak electron acceptor of CKX1. The oxidation of DIMBOA and coniferron generates transitional free radicals that are used by CKX1 as effective electron acceptors. hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-glucoside is not active as an electron acceptor for isozyme CKX1 | Zea mays | ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical + H2O | - |
Zea mays | adenine + 3-methylbut-2-enal + ? | - |
? | |
N6-(2-isopentenyl)adenine + 2,3-dimethoxy-5-methyl-1,4-benzoquinone + H2O | 4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX | Zea mays | adenine + 3-methylbut-2-enal + 2,3-dimethoxy-5-methyl-1,4-benzoquinol | - |
? | |
N6-(2-isopentenyl)adenine + 4-nitrosoresorcinol-1-monomethyl ether + H2O | 4-nitrosoresorcinol-1-monomethyl ether serves as a weak electron acceptor of CKX | Zea mays | adenine + 3-methylbut-2-enal + reduced 4-nitrosoresorcinol-1-monomethyl ether | - |
? | |
N6-(2-isopentenyl)adenine + hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one + H2O | CKX1 is able to use hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one as a poor electron acceptor at neutral pH levels, but not in acidic pH levels | Zea mays | adenine + 3-methylbut-2-enal + reduced hydroxamic acid 2,4-dihydroxy-7-methoxy-1,4-benzoxazin-one | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CKX1 | - |
Zea mays |
cytokinin dehydrogenase | - |
Zea mays |