Application | Comment | Organism |
---|---|---|
medicine | hyperlysinemia | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(NH4)2SO4 | 1 mM, 71% inhibition | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | Homo sapiens | lysine catabolism | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
9.6fold partial purification from liver | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | low enzyme concentration | Homo sapiens | - |
brain | low enzyme concentration | Homo sapiens | - |
heart | high enzyme concentration | Homo sapiens | - |
kidney | after liver next highest enzyme concentration | Homo sapiens | - |
liver | highest enzyme concentration | Homo sapiens | - |
additional information | trace enzyme activity in lung, spleen, and striated muscle, no activity in pancreas, thymus, peripheral leucocyte or erythrocyte | Homo sapiens | - |
skin | low enzyme concentration | Homo sapiens | - |
thyroid gland | low enzyme concentration | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-25°C, 1 month, stable | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | very strict substrate specificity for L-lysine, 2-oxoglutarate and NADPH | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | epsilon-N-(L-glutaryl-2)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | enzyme catalyzes lysine degradation to saccharopine | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine | ? | |
L-lysine + 2-oxoglutarate + NADPH | lysine catabolism | Homo sapiens | N6-(L-1,3-dicarboxypropyl)-L-lysine + NADP+ + H2O | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | 7 | - |
Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.6 | pH 6.2: about 50% of activity maximum, pH 8.6: about 60% of activity maximum | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | NADH can not replace NADPH | Homo sapiens | |
NADPH | specific for NADPH | Homo sapiens |