General Stability | Organism |
---|---|
bovine serum albumin stabilizes | Saccharomyces cerevisiae |
unstable at enzyme concentration below 0.1 mg protein/ml | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetate | NAD+ and NADH protect | Saccharomyces cerevisiae | |
p-chloromercuribenzoate | - |
Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | 40000 | gel filtration, sedimentation equilibrium centrifugation, SDS-PAGE | Saccharomyces cerevisiae |
39000 | - |
1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
baker's yeast | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.09 | - |
crude extract | Saccharomyces cerevisiae |
106 | - |
after purification | Saccharomyces cerevisiae |
Storage Stability | Organism |
---|---|
-20°C, protein concentration above 0.1 mg/ml, pH 6.8, 1 mM EDTA, several months | Saccharomyces cerevisiae |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces cerevisiae |