Application | Comment | Organism |
---|---|---|
environmental protection | strain X1 Fre can effectively dehalogenate dihalophenols, which can be useful for the treatment of dihalophenols in wastewaters and remediation of DCP-contaminated environments | Cupriavidus nantongensis |
Cloned (Comment) | Organism |
---|---|
gene fre, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Cupriavidus nantongensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus nantongensis | - |
- |
- |
Cupriavidus nantongensis X1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme Fre from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration | Cupriavidus nantongensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview | Cupriavidus nantongensis | ? | - |
- |
|
additional information | Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview | Cupriavidus nantongensis X1 | ? | - |
- |
|
additional information | Fre can effectively dehalogenate dihalophenols. Degradation of dichlorophenol (DCP) structural analogues by recombinant 2,4,6-trichlorophenol monooxygenase (TcpA) with NAD(P)H:FAD reductase (Fre), substrate specificities with 2,3-dichlorophenol (2,3-DCP), 2,4-dichlorophenol (2,4-DCP), 2,5-dichlorophenol (2,5-DCP), 2,6-dichlorophenol (2,6-DCP), 3,4-dichlorophenol (3,4 DCP), and 3,5-dichlorophenol (3,5-DCP), overview | Cupriavidus nantongensis X1T | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
fre | - |
Cupriavidus nantongensis |
NAD(P)H:FAD reductase | - |
Cupriavidus nantongensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Cupriavidus nantongensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Cupriavidus nantongensis |
General Information | Comment | Organism |
---|---|---|
additional information | the structure and active site of Fre is constructed and analyzed via homologous modeling and molecular docking | Cupriavidus nantongensis |