BRENDA - Enzyme Database
show all sequences of 1.5.1.37

Characterization of chlorophenol 4-monooxygenase (TftD) and NADH:flavin adenine dinucleotide oxidoreductase (TftC) of Burkholderia cepacia AC1100

Gisi, M.R.; Xun, L.; J. Bacteriol. 185, 2786-2792 (2003)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli as a C-terminally His-tagged fusion protein
Paraburkholderia phenoliruptrix
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0048
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.0103
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.0401
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.164
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, His-tagged TftC, SDS-PAGE
Paraburkholderia phenoliruptrix
Organism
Organism
UniProt
Commentary
Textmining
Paraburkholderia phenoliruptrix
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Paraburkholderia phenoliruptrix
Storage Stability
Storage Stability
Organism
-80°C, His-tagged TftC loses about 50% activity after 2 months
Paraburkholderia phenoliruptrix
0°C, His-tagged TftC is unstable in the elution buffer containing 140 mM imidazole on ice, losing about 50% activity in 2 h
Paraburkholderia phenoliruptrix
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
FAD + NADH + H+
FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate
715349
Paraburkholderia phenoliruptrix
FADH2 + NAD+
-
-
-
?
FMN + NADH + H+
FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate
715349
Paraburkholderia phenoliruptrix
FMNH2 + NAD+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 22000, His-tagged TftC, SDS-PAGE
Paraburkholderia phenoliruptrix
Synonyms
Synonyms
Commentary
Organism
NADH-dependent FAD reductase
-
Paraburkholderia phenoliruptrix
NADH-FAD reductase
-
Paraburkholderia phenoliruptrix
NADH:FAD oxidoreductase
-
Paraburkholderia phenoliruptrix
NADH:flavin adenine dinucleotide oxidoreductase
-
Paraburkholderia phenoliruptrix
TftC
-
Paraburkholderia phenoliruptrix
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
assay at
Paraburkholderia phenoliruptrix
30
-
-
Paraburkholderia phenoliruptrix
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
23
45
23°C: 85% of maximal activity, 45°C: 70% of maximal activity
Paraburkholderia phenoliruptrix
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.9
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
16.6
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
18.5
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
30.1
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
Na-phosphate buffer
Paraburkholderia phenoliruptrix
7
-
assay at
Paraburkholderia phenoliruptrix
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5
7.6
pH 5.0 (sodium succinate buffer): 52% of maximal activity, pH 7.6: 75% of maximal activity
Paraburkholderia phenoliruptrix
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
the enzyme does not use NADPH as substrate
Paraburkholderia phenoliruptrix
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli as a C-terminally His-tagged fusion protein
Paraburkholderia phenoliruptrix
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
the enzyme does not use NADPH as substrate
Paraburkholderia phenoliruptrix
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0048
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.0103
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.0401
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
0.164
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
22000
-
x * 22000, His-tagged TftC, SDS-PAGE
Paraburkholderia phenoliruptrix
Purification (Commentary) (protein specific)
Commentary
Organism
-
Paraburkholderia phenoliruptrix
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, His-tagged TftC loses about 50% activity after 2 months
Paraburkholderia phenoliruptrix
0°C, His-tagged TftC is unstable in the elution buffer containing 140 mM imidazole on ice, losing about 50% activity in 2 h
Paraburkholderia phenoliruptrix
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
FAD + NADH + H+
FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate
715349
Paraburkholderia phenoliruptrix
FADH2 + NAD+
-
-
-
?
FMN + NADH + H+
FAD is a better substrate than FMN. The enzyme does not use NADPH or riboflavin as substrate
715349
Paraburkholderia phenoliruptrix
FMNH2 + NAD+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 22000, His-tagged TftC, SDS-PAGE
Paraburkholderia phenoliruptrix
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
24
-
assay at
Paraburkholderia phenoliruptrix
30
-
-
Paraburkholderia phenoliruptrix
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
23
45
23°C: 85% of maximal activity, 45°C: 70% of maximal activity
Paraburkholderia phenoliruptrix
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
11.9
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
16.6
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
18.5
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
30.1
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
Na-phosphate buffer
Paraburkholderia phenoliruptrix
7
-
assay at
Paraburkholderia phenoliruptrix
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5
7.6
pH 5.0 (sodium succinate buffer): 52% of maximal activity, pH 7.6: 75% of maximal activity
Paraburkholderia phenoliruptrix
General Information
General Information
Commentary
Organism
physiological function
TftD supplies FADH2 to (FADH2)-utilizing monooxygenase TftD, that converts 2,4,5-trichlorophenol to 2,5-dichloro-p-quinol and then to 5-chlorohydroxyquinol. It also converts 2,4,6-trichlorophenol to 2,6-dichloro-p-quinol as the final product
Paraburkholderia phenoliruptrix
General Information (protein specific)
General Information
Commentary
Organism
physiological function
TftD supplies FADH2 to (FADH2)-utilizing monooxygenase TftD, that converts 2,4,5-trichlorophenol to 2,5-dichloro-p-quinol and then to 5-chlorohydroxyquinol. It also converts 2,4,6-trichlorophenol to 2,6-dichloro-p-quinol as the final product
Paraburkholderia phenoliruptrix
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
72.5
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
77.3
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
1796
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
3458
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
72.5
-
NADH
cosubstrate: FMN, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
77.3
-
NADH
cosubstrate: FAD, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
1796
-
FMN
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
3458
-
FAD
cosubstrate: NADH, pH 7.0, 24°C
Paraburkholderia phenoliruptrix
Other publictions for EC 1.5.1.37
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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2
1
2
1
-
-
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1
-
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2
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-
-
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1
2
-
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-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
2
1
1
-
-
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1
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Burkholderia cepacia
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1
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1
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1
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2
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1
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6
1
2
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5
5
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5
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2
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1
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5
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Characterization of chlorophen ...
Paraburkholderia phenoliruptrix
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2003
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1
-
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-
-
4
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-
5
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1
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-
2
2
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5
2
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4
2
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4
-
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1
-
-
-
-
1
-
-
-
2
2
1
2
1
-
4
2
1
-
-
-
1
1
-
4
4