Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron Marine | azo dye, competitive to NADPH and FMN | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
FMN | pH 7.5, 37°C | Bacillus subtilis | |
0.55 | - |
NADPH | pH 7.5, 37°C | Bacillus subtilis | |
0.65 | - |
NADH | pH 7.5, 37°C | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21000 | - |
4 * 21000, SDS-PAGE | Bacillus subtilis |
76000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
enzyme additionally has azoreductase activity | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
reduced riboflavin + NADP+ = riboflavin + NADPH + H+ | bi-bi ternary mechanism, i.e. both FMN and NADPH must bind to the active site before catalysis can occur | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
FMN + NADH | preference for NADPH over NADH, rate of reduction is 80 times faster with NADPH | Bacillus subtilis | FMNH2 + NADP+ | - |
? | |
FMN + NADPH | preference for NADPH over NADH, rate of reduction is 80 times faster with NADPH | Bacillus subtilis | FMNH2 + NADP+ | - |
? | |
additional information | enzyme additionally has azoreductase activity cleaving the -N=N- bond in azo dyes | Bacillus subtilis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 21000, SDS-PAGE | Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
86.5 | - |
melting point of protein | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
Cibacron Marine | pH 7.5, 37°C | Bacillus subtilis | |
60 | - |
FMN | pH 7.5, 37°C | Bacillus subtilis | |
70 | - |
NADPH | pH 7.5, 37°C | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | noncovalently bound | Bacillus subtilis | |
NADH | - |
Bacillus subtilis | |
NADPH | - |
Bacillus subtilis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | - |
Cibacron Marine | substrate FMN, pH 7.5, 37°C | Bacillus subtilis | |
0.015 | - |
Cibacron Marine | substrate NADPH, pH 7.5, 37°C | Bacillus subtilis |