Application | Comment | Organism |
---|---|---|
biotechnology | in vivo screening system to select for functionally active proteins with increased solubility. Fusion of enzyme to green fluorescent protein as reporter for solubility | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
K27E | kinetic properties similar to wild-type, increase in solubility | Plasmodium falciparum |
Y35G/F37L | kinetic properties similar to wild-type, increase in solubility | Plasmodium falciparum |
Y35L/F37R | kinetic properties similar to wild-type, increase in solubility | Plasmodium falciparum |
Y35L/F37T | kinetic properties similar to wild-type, increase in solubility | Plasmodium falciparum |
Y35Q/F37R | kinetic properties similar to wild-type, increase in solubility | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloguanil | - |
Plasmodium falciparum | |
pyrimethamine | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
7,8-dihydrofolate | wild-type, pH 5.0, 25°C | Plasmodium falciparum | |
0.0043 | - |
NADPH | mutant Y35G/F37L, pH 5.0, 25°C | Plasmodium falciparum | |
0.0048 | - |
NADPH | mutant Y35L/F37T, pH 5.0, 25°C | Plasmodium falciparum | |
0.0049 | - |
7,8-dihydrofolate | mutant Y35G/F37L, pH 5.0, 25°C | Plasmodium falciparum | |
0.0053 | - |
7,8-dihydrofolate | mutant K27E, pH 5.0, 25°C | Plasmodium falciparum | |
0.0055 | - |
7,8-dihydrofolate | mutant Y35L/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0058 | - |
NADPH | wild-type, pH 5.0, 25°C | Plasmodium falciparum | |
0.0068 | - |
NADPH | mutant K27E, pH 5.0, 25°C | Plasmodium falciparum | |
0.0076 | - |
7,8-dihydrofolate | mutant Y35Q/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0086 | - |
7,8-dihydrofolate | mutant Y35L/F37T, pH 5.0, 25°C | Plasmodium falciparum | |
0.0097 | - |
NADPH | mutant Y35L/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0105 | - |
NADPH | mutant Y35Q/F37R, pH 5.0, 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | P13922 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type enzyme readily aggregates at 2 mg/ml | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Plasmodium falciparum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000012 | - |
pyrimethamine | mutant K27E, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000002 | - |
pyrimethamine | mutant Y35G/F37L, pH 5.0, 25°C | Plasmodium falciparum | |
0.00000024 | - |
cycloguanil | mutant K27E, pH 5.0, 25°C | Plasmodium falciparum | |
0.00000028 | - |
cycloguanil | mutant Y35G/F37L, pH 5.0, 25°C | Plasmodium falciparum | |
0.00000057 | - |
pyrimethamine | mutant Y35L/F37T, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000006 | - |
cycloguanil | mutant Y35Q/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000006 | - |
pyrimethamine | wild-type, pH 5.0, 25°C | Plasmodium falciparum | |
0.00000061 | - |
pyrimethamine | mutant Y35Q/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000012 | - |
pyrimethamine | mutant Y35L/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000012 | - |
cycloguanil | mutant Y35L/F37R, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000015 | - |
cycloguanil | wild-type, pH 5.0, 25°C | Plasmodium falciparum | |
0.0000018 | - |
cycloguanil | mutant Y35L/F37T, pH 5.0, 25°C | Plasmodium falciparum |