Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
diffraction to 3.5 A resolution | Saccharolobus solfataricus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Saccharolobus solfataricus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
x * 30000, SDS-PAGE. Enzyme self-associates to form large multimeric complexes. The most stable multimeric configuration is a decamer, which can further self-associate to form higher order complexes | Saccharolobus solfataricus |
320000 | - |
gel filtration | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q97ZT3 | - |
- |
Saccharolobus solfataricus P2 | Q97ZT3 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-pyrroline-5-carboxylate + NAD(P)H | - |
Saccharolobus solfataricus | L-proline + NAD(P)+ | - |
r | |
1-pyrroline-5-carboxylate + NAD(P)H | - |
Saccharolobus solfataricus P2 | L-proline + NAD(P)+ | - |
r | |
L-proline + NAD(P)+ | - |
Saccharolobus solfataricus | 1-pyrroline-5-carboxylate + NAD(P)H | - |
r | |
L-proline + NAD(P)+ | - |
Saccharolobus solfataricus P2 | 1-pyrroline-5-carboxylate + NAD(P)H | - |
r | |
L-thioproline + NAD(P)+ | - |
Saccharolobus solfataricus | 1-pyrroline-3-thio-5-carboxylate + NAD(P)H | - |
r | |
L-thioproline + NAD(P)+ | - |
Saccharolobus solfataricus P2 | 1-pyrroline-3-thio-5-carboxylate + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
multimer | x * 30000, SDS-PAGE. Enzyme self-associates to form large multimeric complexes. The most stable multimeric configuration is a decamer, which can further self-associate to form higher order complexes | Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
stable for at least 30 min | Saccharolobus solfataricus |
80 | - |
half-life 30 min | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
reaction rate at pH 9.0 is nearly 3fold higher than at pH 7.0 | Saccharolobus solfataricus |
9 | - |
reaction rate is nearly 3fold higher than at pH 7.0 | Saccharolobus solfataricus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | 2fold preference for NADP+ with apparent KM 0.172 mM over NAD+ with apparent KM 0.3896 mM | Saccharolobus solfataricus | |
NADH | - |
Saccharolobus solfataricus | |
NADP+ | 2fold preference for NADP+ with apparent KM 0.172 mM over NAD+ with apparent KM 0.3896 mM | Saccharolobus solfataricus | |
NADPH | - |
Saccharolobus solfataricus |