Activating Compound | Comment | Organism | Structure |
---|---|---|---|
phosphate | absolutely required, binds in close proximity to the 2-hydroxyl of NAD through interactions with R166 and R198. Km-value of wild-type 0.412 mM | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
construction of homology model | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D133A | cosubstrate NAD+, 1.7% of wild-type activity, cosubstrate NADP+, 5.5% of wild-type activity | Homo sapiens |
D133E | no enzymic activity | Homo sapiens |
D133N | cosubstrate NAD+, 20.4% of wild-type activity, cosubstrate NADP+, 82.2% of wild-type activity | Homo sapiens |
D133S | cosubstrate NAD+, 17.8% of wild-type activity, cosubstrate NADP+, 34.5% of wild-type activity | Homo sapiens |
D190A | no enzymic activity | Homo sapiens |
D190E | cosubstrate NAD+, 1% of wild-type activity | Homo sapiens |
D190N | cosubstrate NAD+, 16% of wild-type activity | Homo sapiens |
D190S | no enzymic activity | Homo sapiens |
R166A | no enzymic activity | Homo sapiens |
R166K | no enzymic activity | Homo sapiens |
R166S | no enzymic activity | Homo sapiens |
R198A | cosubstrate NAD+, 0.7% of wild-type activity, cosubstrate NADP+, 5.3% of wild-type activity | Homo sapiens |
R198K | cosubstrate NAD+, 56% of wild-type activity, cosubstrate NADP+, 42% of wild-type activity | Homo sapiens |
R198S | cosubstrate NAD+, 0.9% of wild-type activity, cosubstrate NADP+, 53% of wild-type activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.202 | - |
NAD+ | wild-type, pH 7.3 | Homo sapiens | |
0.255 | - |
NADP+ | mutant R198S, pH 7.3 | Homo sapiens | |
0.352 | - |
NADP+ | wild-type, pH 7.3 | Homo sapiens | |
0.503 | - |
NADP+ | mutant D133S, pH 7.3 | Homo sapiens | |
0.58 | - |
NADP+ | mutant D133N, pH 7.3 | Homo sapiens | |
0.689 | - |
NADP+ | mutant D190N, pH 7.3 | Homo sapiens | |
0.918 | - |
NAD+ | mutant R198K, pH 7.3 | Homo sapiens | |
1.99 | - |
NAD+ | mutant D133N, pH 7.3 | Homo sapiens | |
2.12 | - |
NADP+ | mutant R198K, pH 7.3 | Homo sapiens | |
2.17 | - |
NAD+ | mutant D133S, pH 7.3 | Homo sapiens | |
3.88 | - |
NAD+ | mutant D190N, pH 7.3 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P13995 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.024 | - |
mutant D190E, cosubstrate NAD+, pH 7.3 | Homo sapiens |
0.156 | - |
mutant R198A, cosubstrate NADP+, pH 7.3 | Homo sapiens |
0.16 | - |
mutant D133A, cosubstrate NADP+, pH 7.3 | Homo sapiens |
0.17 | - |
mutant R198A, cosubstrate NAD+, pH 7.3 | Homo sapiens |
0.207 | - |
mutant R198S, cosubstrate NAD+, pH 7.3 | Homo sapiens |
0.392 | - |
mutant D133A, cosubstrate NAD+, pH 7.3 | Homo sapiens |
1.01 | - |
mutant D133S, cosubstrate NADP+, pH 7.3 | Homo sapiens |
1.24 | - |
mutant R198K, cosubstrate NADP+, pH 7.3 | Homo sapiens |
1.54 | - |
mutant R198S, cosubstrate NADP+, pH 7.3 | Homo sapiens |
2 | 2.5 | wild-type, cosubstrate NAD+, pH 7.3 | Homo sapiens |
2.4 | - |
mutant D133N, cosubstrate NADP+, pH 7.3 | Homo sapiens |
2.92 | - |
wild-type, cosubstrate NADP+, pH 7.3 | Homo sapiens |
3.64 | - |
mutant D190N, cosubstrate NAD+, pH 7.3 | Homo sapiens |
3.96 | - |
mutant D190N, cosubstrate NADP+, pH 7.3 | Homo sapiens |
3.99 | - |
mutant D133S, cosubstrate NAD+, pH 7.3 | Homo sapiens |
4.6 | - |
mutant D133N, cosubstrate NAD+, pH 7.3 | Homo sapiens |
12.6 | - |
mutant R198K, cosubstrate NAD+, pH 7.3 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NAD+ | - |
Homo sapiens | 5,10-methenyltetrahydrofolate + NADH | - |
? | |
5,10-methylenetetrahydrofolate + NADP+ | about 15% of the rate with NAD+ | Homo sapiens | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |