Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
beta-phenylethylamine | substrate inhibition | Pseudomonas sp. | |
hydroxylamine | - |
Pseudomonas sp. | |
phenylhydrazine | - |
Pseudomonas sp. | |
Semicarbazide | - |
Pseudomonas sp. | |
tryptamine | substrate inhibition | Pseudomonas sp. | |
tyramine | substrate inhibition | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
tyramine | - |
Pseudomonas sp. | |
0.0045 | - |
2-Phenylethylamine | - |
Pseudomonas sp. | |
0.0092 | - |
tryptamine | - |
Pseudomonas sp. | |
0.11 | - |
phenazine methosulfate | - |
Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
copper | metal content: 0.1 mol per mol of enzyme | Pseudomonas sp. | |
Iron | metal content: 0.6 mol per mol of enzyme | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
8000 | - |
alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE | Pseudomonas sp. |
46000 | - |
alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE | Pseudomonas sp. |
103000 | - |
sedimentation equilibrium | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.11 | - |
after purification | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + 2,6-dichlorophenolindophenol | - |
Pseudomonas sp. | phenylacetaldehyde + NH3 + reduced 2,6-dichlorophenolindophenol | - |
r | |
dopamine + acceptor + H2O | - |
Pseudomonas sp. | 4-(2-oxoethyl)-1,2-benzenediol + NH3 + reduced acceptor | - |
? | |
additional information | long-chain aliphatic amines, phenazine methosulfate is the only effective electron acceptor | Pseudomonas sp. | ? | - |
? | |
additional information | not: methylamine, ethylamine, polyamines | Pseudomonas sp. | ? | - |
? | |
additional information | slow reaction with aromatic amines | Pseudomonas sp. | ? | - |
? | |
n-hexylamine + acceptor + H2O | - |
Pseudomonas sp. | hexanal + NH3 + reduced acceptor | - |
? | |
n-octylamine + acceptor + H2O | - |
Pseudomonas sp. | octanal + NH3 + reduced acceptor | - |
? | |
phenazine methosulfate + H2O + 2,6-dichlorophenol indophenol | - |
Pseudomonas sp. | ? + NH3 + reduced 2,6-dichlorophenol indophenol | - |
? | |
serotonin + acceptor + H2O | - |
Pseudomonas sp. | 3-(2-oxoethyl)-1H-indol-5-ol + NH3 + reduced acceptor | - |
? | |
tryptamine + acceptor + H2O | - |
Pseudomonas sp. | 1H-indole-3-acetaldehyde + NH3 + reduced acceptor | - |
? | |
tyramine + acceptor + H2O | - |
Pseudomonas sp. | 4-hydroxyphenylacetaldehyde + NH3 + reduced acceptor | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | alpha2beta2, 2 * 46000 + 2 * 8000, SDS-PAGE | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
tyramine, beta-phenylethylamine | Pseudomonas sp. |
8 | - |
serotonin, tryptamine | Pseudomonas sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.5 | 5.5: about 25% of activity maximum, 9.5: about 10% of activity maximum | Pseudomonas sp. |