Cloned (Comment) | Organism |
---|---|
expression in Saccharomyces cerevisiae BJ 2168 | Rattus norvegicus |
expression in yeast | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y402A | decrease in activity not significant, decrease in the FAD incorporation | Rattus norvegicus |
Y402F | decrease in activity not significant | Rattus norvegicus |
Y402F | the ratio of turnover number to Km-value for serotonin is 3.4fold lower than that of the mutant enzyme, the ratio of turnover number to Km-value for phenylethylamine is 3.3fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for tyramine is 2.1fold lower than that of the wild-type enzyme, the ratio of turnover number to KM-value for tryptamine is 2.1fold lower than that of the wild-type enzyme | Rattus norvegicus |
Y403A | the ratio of turnover number to Km-value for serotonin is 95.6fold lower than that of the mutant enzyme, the ratio of turnover number to Km-value for phenylethylamine is 210fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for tyramine is 170fold lower than that of the wild-type enzyme, the ratio of turnover number to KM-value for tryptamine is 91fold lower than that of the wild-type enzyme. Mutant enzyme is sensitive to trypsin treatment, while the wild-type enzyme is resistant | Rattus norvegicus |
Y407A | no activity with serotonin, phenylethylamine, tyramine, low activity with tryptamine, decrease in the FAD incorporation | Rattus norvegicus |
Y407A | mutation leads to almost complete loss of catalytic activity for serotonin, phenylethylamine, tyramine and tryptamine. Mutant enzyme is sensitive to trypsin treatment, while the wild-type enzyme is resistant | Rattus norvegicus |
Y407F | little changes in Km for all substrates | Rattus norvegicus |
Y407F | the ratio of turnover number to Km-value for serotonin is 2.6fold lower than that of the mutant enzyme, the ratio of turnover number to Km-value for phenylethylamine is 4.3fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for tyramine is 2.7fold lower than that of the wild-type enzyme, the ratio of turnover number to KM-value for tryptamine is 1.3fold lower than that of the wild-type enzyme | Rattus norvegicus |
Y410A | decrease in activity not significant | Rattus norvegicus |
Y410A | the ratio of turnover number to Km-value for serotonin is 7.8fold lower than that of the mutant enzyme, the ratio of turnover number to Km-value for phenylethylamine is 6fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for tyramine is 3.4fold lower than that of the wild-type enzyme, the ratio of turnover number to KM-value for tryptamine is 3.4fold lower than that of the wild-type enzyme. Mutant enzyme is sensitive to trypsin treatment, while the wild-type enzyme is resistant | Rattus norvegicus |
General Stability | Organism |
---|---|
wild-type enzyme is resistant to trypsin treatment, mutant enzyms Y403A, Y407A and Y410A are sensitive | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | comparison of Km of mutant enzymes using serotonin, phenylethylamine, tyramine, tryptamine as substrates | Rattus norvegicus | |
0.028 | - |
tryptamine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
0.033 | - |
tryptamine | 30°C, wild-type enzyme | Rattus norvegicus | |
0.035 | - |
tryptamine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
0.038 | - |
tryptamine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
0.055 | - |
tryptamine | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.44 | - |
serotonin | 30°C, wild-type enzyme | Rattus norvegicus | |
0.51 | - |
serotonin | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
0.55 | - |
serotonin | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.57 | - |
2-Phenylethylamine | 30°C, wild-type enzyme | Rattus norvegicus | |
0.59 | - |
serotonin | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
0.6 | - |
tyramine | 30°C, wild-type enzyme | Rattus norvegicus | |
0.63 | - |
tyramine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
0.78 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
0.95 | - |
serotonin | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
1.13 | - |
tyramine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
1.13 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
1.42 | - |
tyramine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
1.42 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
1.98 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
3.1 | - |
tryptamine | 30°C, mutant enzyme Y407A | Rattus norvegicus | |
4.6 | - |
tyramine | 30°C, mutant enzyme Y402A | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrial outer membrane | - |
Rattus norvegicus | 5741 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
RCH2NH2 + H2O + O2 | Rattus norvegicus | - |
RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | Rattus norvegicus MAO-A | - |
RCHO + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | - |
MAO-A | - |
Rattus norvegicus MAO-A | - |
MAO-A | - |
Purification (Comment) | Organism |
---|---|
His-tagged rat enzyme and its mutants Y403A, Y402F, Y407A, Y407F, Y410A | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-phenylethylamine + H2O + O2 | - |
Rattus norvegicus | 2-phenylethanal + NH3 + H2O2 | - |
? | |
2-phenylethylamine + H2O + O2 | - |
Rattus norvegicus MAO-A | 2-phenylethanal + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | - |
Rattus norvegicus | RCHO + NH3 + H2O2 | - |
? | |
RCH2NH2 + H2O + O2 | - |
Rattus norvegicus MAO-A | RCHO + NH3 + H2O2 | - |
? | |
serotonin + H2O + O2 | - |
Rattus norvegicus | (5-hydroxy-1H-indol-3-yl)acetaldehyde + NH3 + H2O2 | - |
? | |
tryptamine + H2O + O2 | - |
Rattus norvegicus | 1-H-indol-3-yl-acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Rattus norvegicus | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? | |
tyramine + H2O + O2 | - |
Rattus norvegicus MAO-A | (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MAOA | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.066 | - |
tryptamine | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.17 | - |
serotonin | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.183 | - |
tryptamine | 30°C, mutant enzyme Y407A | Rattus norvegicus | |
0.33 | - |
tyramine | 30°C, mutant enzyme Y402A | Rattus norvegicus | |
0.5 | - |
serotonin | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
0.67 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
0.833 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
1.17 | - |
2-Phenylethylamine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
1.2 | - |
tryptamine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
1.267 | - |
serotonin | 30°C, wild-type enzyme | Rattus norvegicus | |
1.8 | - |
tryptamine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
1.83 | - |
serotonin | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
2 | - |
2-Phenylethylamine | 30°C, wild-type enzyme | Rattus norvegicus | |
2 | - |
tyramine | 30°C, mutant enzyme Y410A | Rattus norvegicus | |
2.4 | - |
tryptamine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
3.33 | - |
tyramine | 30°C, mutant enzyme Y402F | Rattus norvegicus | |
3.5 | - |
tryptamine | 30°C, wild-type enzyme | Rattus norvegicus | |
4.83 | - |
tyramine | 30°C, mutant enzyme Y407F | Rattus norvegicus | |
6.83 | - |
tyramine | 30°C, wild-type enzyme | Rattus norvegicus | |
10.67 | - |
serotonin | 30°C, mutant enzyme Y407F | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the attachment to the enzyme may require a pocket constructed partially of tyrosine residues. Through the interactions among the aromatic rings of the tyrosine residues and that of FAD via pi-pi cloud, the enzyme forms a catalytic center and adopts a stable conformation | Rattus norvegicus | |
flavin | tyrosine residues near Cys406 may form a pocket to facilitate FAD incorporation and a stable conformation, probably through interactions among the aromatic rings of the tyrosine residues and FAD | Rattus norvegicus |