Protein Variants | Comment | Organism |
---|---|---|
additional information | immobilization of the enzyme onto magnetic nanoparticles stabilizes the enzyme | Rhodotorula toruloides |
General Stability | Organism |
---|---|
stabilization of the enzyme by immobilization onto Fe3O4 magnetic nanoparticles, after immobilization the Tm increases from 45°C of the free form to 55°C. In the presence of 20 mM H2O2, the immobilized form retains 93% of its activity after 5 h while the free form is completely inactivated after 3.5 h | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-alanine | free enzyme | Rhodotorula toruloides | |
1 | - |
D-alanine | immobilized enzyme | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
27 | - |
immobiliuzed enzyme | Rhodotorula toruloides |
59 | - |
free enzyme | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
free enzyme | Rhodotorula toruloides |
50 | - |
immobilized enzyme | Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.8 | - |
D-alanine | immobilized enzyme | Rhodotorula toruloides | |
33.8 | - |
D-alanine | free enzyme | Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | free and immobilized enzymes, stable, profile, overview | Rhodotorula toruloides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13.8 | - |
D-alanine | immobilized enzyme | Rhodotorula toruloides | |
49.7 | - |
D-alanine | free enzyme | Rhodotorula toruloides |