General Stability | Organism |
---|---|
2-mercaptoethanol stabilizes | Rhodotorula toruloides |
changes in ionic strength, I: 16-82 mM, have only moderate effect on enzyme stability, stability maximum is 50 mM | Rhodotorula toruloides |
glycerol, 10%, stabilizes | Rhodotorula toruloides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1,3-Butadien 1-carboxylic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
2-hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
2-hydroxybutyric acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
2-ketobutyric acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
3-Bromobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
3-chlorobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
3-Hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
3-methylbenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
3-Nitrobenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
4-hydroxybenzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
benzoic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
crotonic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
kojic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
nicotinic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
Picolinic acid | D-phenylglycine oxidation | Rhodotorula toruloides | |
trans-2-Pentenoic acid | D-phenylglycine oxidation | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
O2 | with D-methionine | Rhodotorula toruloides | |
0.29 | - |
D-phenylalanine | - |
Rhodotorula toruloides | |
0.3 | - |
D-tryptophan | - |
Rhodotorula toruloides | |
0.39 | - |
D-norleucine | - |
Rhodotorula toruloides | |
0.5 | - |
D-leucine | - |
Rhodotorula toruloides | |
0.83 | - |
D-methionine | - |
Rhodotorula toruloides | |
0.89 | - |
D-Norvaline | - |
Rhodotorula toruloides | |
13.75 | - |
D-serine | - |
Rhodotorula toruloides | |
18.89 | - |
D-valine | - |
Rhodotorula toruloides | |
21.41 | - |
D-cysteine | - |
Rhodotorula toruloides | |
21.47 | - |
D-proline | - |
Rhodotorula toruloides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | Rhodotorula toruloides | - |
pyruvic acid + NH3 + H2O2 | - |
? | |
D-cysteine + H2O + O2 | Rhodotorula toruloides | - |
2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
D-leucine + H2O + O2 | Rhodotorula toruloides | - |
4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | Rhodotorula toruloides | - |
4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-norleucine + H2O + O2 | Rhodotorula toruloides | - |
2-oxohexanoate + NH3 + H2O2 | - |
? | |
D-norvaline + H2O + O2 | Rhodotorula toruloides | - |
2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | Rhodotorula toruloides | - |
phenylpyruvic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | Rhodotorula toruloides | - |
2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | Rhodotorula toruloides | - |
2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
D-tryptophan + H2O + O2 | Rhodotorula toruloides | - |
indol-3-pyruvic acid + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | Rhodotorula toruloides | - |
alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
thiazolidine-2-carboxylic acid + H2O + O2 | Rhodotorula toruloides | - |
? + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a D-amino acid + H2O + O2 = a 2-oxo carboxylate + NH3 + H2O2 | ping pong bi bi mechanism | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Rhodotorula toruloides | pyruvic acid + NH3 + H2O2 | - |
? | |
D-cysteine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-thiopropionic acid + NH3 + H2O2 | - |
? | |
D-leucine + H2O + O2 | - |
Rhodotorula toruloides | 4-methyl-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-methionine + H2O + O2 | - |
Rhodotorula toruloides | 4-methylthio-2-oxobutanoic acid + NH3 + H2O2 | - |
? | |
D-norleucine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxohexanoate + NH3 + H2O2 | - |
? | |
D-norvaline + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoate + NH3 + H2O2 | - |
? | |
D-phenylalanine + H2O + O2 | - |
Rhodotorula toruloides | phenylpyruvic acid + NH3 + H2O2 | - |
? | |
D-proline + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
D-serine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxo-3-hydroxypropionic acid + NH3 + H2O2 | - |
? | |
D-tryptophan + H2O + O2 | - |
Rhodotorula toruloides | indol-3-pyruvic acid + NH3 + H2O2 | - |
? | |
D-valine + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoisovaleric acid + NH3 + H2O2 | - |
? | |
thiazolidine-2-carboxylic acid + H2O + O2 | - |
Rhodotorula toruloides | ? + NH3 + H2O2 | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Rhodotorula toruloides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 50 | less than 50% of maximal activity above and below | Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
523 | - |
D-valine | - |
Rhodotorula toruloides | |
721 | - |
D-alanine | - |
Rhodotorula toruloides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | - |
Rhodotorula toruloides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.3 | 7.9 | 30 min stable | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Rhodotorula toruloides |