Protein Variants | Comment | Organism |
---|---|---|
M213R | the ratio of turnover number to Km-value for D-Asp is 74fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Glu is 29.5fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Ala is 172fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Pro is 166fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Asn is 9.4fold lower than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Gln is 3.2fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Met is 56fold lower than that of the wild-type enzyme | Rhodotorula toruloides |
M213R/Y238R | the ratio of turnover number to Km-value for D-Asp is 36.6fold higher than that of the wild-type enzyme, the ratio of turnover number to Km-value for D-Ala is 1000fold lower than that of the wild-type enzyme | Rhodotorula toruloides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-Met | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
0.8 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1.5 | - |
D-Met | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
2 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
3.8 | - |
D-Gln | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
4.6 | - |
D-Gln | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
11 | - |
D-Asn | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
14.4 | - |
D-Asn | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
17.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
18 | - |
N-Methyl-D-aspartate | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
18 | - |
D-Asp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
21.5 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
28 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
33 | - |
D-Glu | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
45.8 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
77.3 | - |
D-Glu | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1280 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
2 * 40000, mutant enzyme M213R and wilde-type enzyme, SDS-PAGE | Rhodotorula toruloides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula toruloides | P80324 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant enzyme M213R | Rhodotorula toruloides |
Source Tissue | Comment | Organism | Textmining |
---|
Storage Stability | Organism |
---|---|
-20°C, wild-type and mutant enzyme M213R are stable for several months | Rhodotorula toruloides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Ala + H2O + O2 | - |
Rhodotorula toruloides | pyruvate + NH3 + H2O2 | - |
? | |
D-Asn + H2O + O2 | - |
Rhodotorula toruloides | 2-oxosuccinamate + NH3 + H2O2 | - |
? | |
D-Asp + H2O + O2 | - |
Rhodotorula toruloides | oxaloacetate + NH3 + H2O2 | - |
? | |
D-Glu + H2O + O2 | - |
Rhodotorula toruloides | alpha-ketoglutarate + NH3 + H2O2 | - |
? | |
D-glutamine + H2O + O2 | - |
Rhodotorula toruloides | 2-oxoglutaramate + NH3 + H2O2 | - |
? | |
D-Met + H2O + O2 | - |
Rhodotorula toruloides | 4-methylthio-2-oxobutanoate + NH3 + H2O2 | - |
? | |
D-Pro + H2O + O2 | - |
Rhodotorula toruloides | 2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
N-methyl-D-aspartate + H2O + O2 | wild-type enzyme shows no activity, mutant enzyme M213R is active | Rhodotorula toruloides | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, mutant enzyme M213R and wilde-type enzyme, SDS-PAGE | Rhodotorula toruloides |
Synonyms | Comment | Organism |
---|---|---|
DAAO | - |
Rhodotorula toruloides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
29 | - |
D-Asp | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
60 | - |
D-Glu | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
79 | - |
N-Methyl-D-aspartate | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
106 | - |
D-Asn | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
235 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
278 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
290 | - |
D-Gln | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
630 | - |
D-Ala | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
680 | - |
D-Met | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
975 | - |
D-Glu | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1135 | - |
D-Gln | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1300 | - |
D-Asn | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
1615 | - |
D-Pro | pH 8.5, 25°C, mutant enzyme M213R | Rhodotorula toruloides | |
1665 | - |
D-Asp | pH 8.5, 25°C, mutant enzyme M213R/Y238R | Rhodotorula toruloides | |
4600 | - |
D-Met | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
4640 | - |
D-Pro | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides | |
5000 | - |
D-Ala | pH 8.5, 25°C, wild-type enzyme | Rhodotorula toruloides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | the mutant enzyme M213R has a more polar microenvironment surrounding the flavin coenzyme | Rhodotorula toruloides |