Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | strong inhibition | Pseudomonas sp. | |
Co2+ | strong inhibition | Pseudomonas sp. | |
Cu2+ | strong inhibition | Pseudomonas sp. | |
additional information | not inhibitory: Ca2+, Mg2+, Mn2+, Ni2+, Zn2+ | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.247 | - |
4-(methylamino)-1-(pyridin-3-yl)butan-1-one | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54101 | - |
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
55000 | - |
x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | H8ZPX1 | - |
- |
Pseudomonas sp. HZN6 | H8ZPX1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 | - |
Pseudomonas sp. | 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2 | - |
? | |
4-(methylamino)-1-(pyridin-3-yl)butan-1-one + H2O + O2 | - |
Pseudomonas sp. HZN6 | 4-oxo-4-(pyridin-3-yl)butanal + methylamine + H2O2 | - |
? | |
additional information | no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine | Pseudomonas sp. | ? | - |
? | |
additional information | no substrates: nicotine, gamma-N-methylaminobutanoate, gamma-aminobutanoate, spermidine, spermine, sarcosine, methylamine, dimethylamine, dimethylglycine, and 3-(methylamino)propylamine | Pseudomonas sp. HZN6 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 54101, calculated, x * 55000, SDS-PAGE, His-tagged recombinant protein | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
PAO | - |
Pseudomonas sp. |
pseudooxynicotine amine oxidase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
151 | - |
4-(methylamino)-1-(pyridin-3-yl)butan-1-one | pH 8.0, temperature not specified in the publication | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | noncovalently bound, 0.5 mol per mol of subunit | Pseudomonas sp. | |
additional information | no FMN detected | Pseudomonas sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | gene disruption mutant strain is unable to use nicotine as the sole carbon source and accumulates pseudooxynicotine | Pseudomonas sp. |