Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag2+ | 2.0 mM | Bacillus subtilis | |
Cd2+ | 2.0 mM | Bacillus subtilis | |
Cu2+ | 2.0 mM | Bacillus subtilis | |
Hg2+ | 2.0 mM | Bacillus subtilis | |
PCMB | 2.0 mM | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
sarcosine | - |
Bacillus subtilis | |
0.66 | - |
N-ethylglycine | - |
Bacillus subtilis | |
0.99 | - |
Gly | - |
Bacillus subtilis | |
46 | - |
D-Pro | - |
Bacillus subtilis | |
81 | - |
D-Ala | - |
Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
4 * 42000, SDS-PAGE | Bacillus subtilis |
159000 | - |
gel filtration | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme expressed in Escherichia coli | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-aminobutyrate + H2O + O2 | 2.2% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
D-Ala + H2O + O2 | 7.4% of the activity with sarcosine | Bacillus subtilis | pyruvate + NH3 + H2O2 | - |
? | |
D-Pro + H2O + O2 | 15.1% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
D-Val + H2O + O2 | 4.8% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | 77.4% of the activity with sarcosine | Bacillus subtilis | glyoxylate + NH3 + H2O2 | - |
? | |
N-ethylglycine + H2O + O2 | 85.3% of the activity with sarcosine | Bacillus subtilis | glyoxylate + ethylamine + H2O2 | - |
? | |
N-methyl-D-Ala + H2O + O2 | 16.9% of the activity with sarcosine | Bacillus subtilis | ? + NH3 + H2O2 | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus subtilis | glyoxylate + methylamine + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 42000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
5 h, most stable between pH 7.5 and 8.5 | Bacillus subtilis |
45 | - |
10 min, stable up to | Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
D-Ala | - |
Bacillus subtilis | |
1.3 | - |
Gly | - |
Bacillus subtilis | |
1.3 | - |
D-Pro | - |
Bacillus subtilis | |
1.4 | - |
N-ethylglycine | - |
Bacillus subtilis | |
1.6 | - |
sarcosine | - |
Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Bacillus subtilis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | 25°C, 5 h, most stable | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | flavoprotein | Bacillus subtilis |