Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Top10 cells | Rhodococcus erythropolis |
Protein Variants | Comment | Organism |
---|---|---|
E324A | the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme | Rhodococcus erythropolis |
E324L | the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme | Rhodococcus erythropolis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(2-aminoethyl)-trimethylammonium | competitive inhibitor | Rhodococcus erythropolis | |
1,3-diaminopropane | competitive inhibitor | Rhodococcus erythropolis | |
Aminoethanol | competitive inhibitor | Rhodococcus erythropolis | |
ethylenediamine | competitive inhibitor | Rhodococcus erythropolis | |
additional information | short diamines and monoamines strongly inhibit activity | Rhodococcus erythropolis | |
n-butylamine | competitive inhibitor | Rhodococcus erythropolis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0035 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
0.0082 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.018 | - |
cadaverine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.065 | - |
2-hydroxyputrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.1 | - |
spermidine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.17 | - |
1,6-diaminohexane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.33 | - |
L-ornithine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.47 | - |
spermine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
110 | - |
putrescine | mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
110 | - |
putrescine | mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
2 * 54000, SDS-PAGE | Rhodococcus erythropolis |
98750 | - |
theoretical molecular mass | Rhodococcus erythropolis |
108000 | - |
SDS-PAGE | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | B0F9F6 | fragment | - |
Rhodococcus erythropolis NCIMB 11540 | B0F9F6 | fragment | - |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,6-diaminohexane + O2 + H2O | 1.4% activity compared to putrescine | Rhodococcus erythropolis | 6-aminohexanal + NH3 + H2O2 | - |
? | |
2-hydroxyputrescine + O2 + H2O | 28% activity compared to putrescine | Rhodococcus erythropolis | ? | - |
? | |
4-amino-1-butanol + O2 + H2O | 0.6% activity compared to putrescine | Rhodococcus erythropolis | 4-hydroxybutanal + NH3 + H2O2 | - |
? | |
cadaverine + O2 + H2O | 14% activity compared to putrescine | Rhodococcus erythropolis | 5-aminopentanal + NH3 + H2O2 | - |
? | |
cadaverine + O2 + H2O | 14% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | 5-aminopentanal + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | 0.08% activity compared to putrescine | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with aromatic amines and amino alcohols | Rhodococcus erythropolis | ? | - |
? | |
additional information | no activity with aromatic amines and amino alcohols | Rhodococcus erythropolis NCIMB 11540 | ? | - |
? | |
putrescine + O2 + H2O | 100% activity | Rhodococcus erythropolis | 4-aminobutanal + NH3 + H2O2 | - |
? | |
putrescine + O2 + H2O | 100% activity | Rhodococcus erythropolis NCIMB 11540 | 4-aminobutanal + NH3 + H2O2 | - |
? | |
spermidine + O2 + H2O | 5.3% activity compared to putrescine | Rhodococcus erythropolis | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
spermidine + O2 + H2O | 5.3% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
spermine + O2 + H2O | 0.7% activity compared to putrescine | Rhodococcus erythropolis | N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2 | - |
? | |
spermine + O2 + H2O | 0.7% activity compared to putrescine | Rhodococcus erythropolis NCIMB 11540 | N,N'-bis(3-aminopropyl)-4-aminobutanal + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 54000, SDS-PAGE | Rhodococcus erythropolis |
Synonyms | Comment | Organism |
---|---|---|
PuO | - |
Rhodococcus erythropolis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 50 | at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0198 | - |
L-ornithine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.18 | - |
spermine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.37 | - |
1,6-diaminohexane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
1.4 | - |
spermidine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
3.2 | - |
putrescine | mutant enzyme E324L, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
3.9 | - |
cadaverine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
6.1 | - |
putrescine | mutant enzyme E324A, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
7.5 | - |
2-hydroxyputrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
20.7 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 9.0, at 25°C | Rhodococcus erythropolis | |
26.4 | - |
putrescine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
around pH 8.0 | Rhodococcus erythropolis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.4 | - |
below pH 6.4, no significant activity can be detected | Rhodococcus erythropolis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | contains non-covalently bound FAD | Rhodococcus erythropolis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
Aminoethanol | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.064 | - |
1,3-diaminopropane | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.12 | - |
n-butylamine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
0.66 | - |
ethylenediamine | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis | |
5.8 | - |
(2-aminoethyl)-trimethylammonium | wild type enzyme, in 50 mM Tris-HCl buffer, pH 8.0, at 25°C | Rhodococcus erythropolis |